孕激素调节HSD11B1介导的牛肉花粉细胞中的皮质醇产生
Memory Mukangwa1, Masafumi Tetsuka1
1Department of Life and Food Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
The Journal of reproduction and development
|June 21, 2023
概括
孕激素,像孕激素一样,通过改变关键酶的表达,降低了牛肉质化颗粒细胞中的皮质醇产生. 这项研究阐明了这些细胞内的复杂类固醇激素相互作用.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- 化颗粒细胞 (LGCs) 对于排卵和类固醇生成至关重要.
- 孕激素 (P4) 和皮质醇的产量增加在LGCs在周卵期期间.
- 在LGC中P4和皮质醇之间的确切相互作用仍然不完全理解.
研究的目的:
- 为了研究孕激素 (P4) 和皮质醇在培养的牛LGCs之间的相互作用.
- 确定P4和皮质醇如何影响对方的合成和基因表达.
- 阐明P4和皮质醇对关键类固醇酶的调节作用.
主要方法:
- 主要的牛LGC被培养长达14天.
- 分析了类固醇的产生和基因表达 (STAR,CYP11A1,HSD3B1,HSD11B1).
- 用P4合成抑制剂 (trilostane),孕激素 (NA),P4和/或皮质醇治疗LGCs.
主要成果:
- 随着培养时间的推移,P4和类固醇酶的表达增加.
- 孕激素 (NA,P4) 的剂量依赖性降低了皮质醇的产生和HSD11B1/HSD3B1的表达.
- 皮质醇增加了HSD11B1/HSD3B1的表达,并减少了STAR的表达,但没有影响P4的产生.
结论:
- 孕激素通过调节HSD11B1表达来抑制牛LGC中的皮质醇产生.
- 孕激素和皮质醇对STAR,HSD3B1和HSD11B1的表达有不同的调节作用.
- 这项研究提供了关于卵巢细胞中类固醇激素交叉的见解.
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