相关实验视频

Updated: Jul 26, 2025

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

Published on: October 15, 2013

阻断免疫检查点LAG-3和PD-1对人类不变的自然杀手T细胞功能的影响

Allison L Balasko¹, Monika M Kowatsch¹, Colin Graydon¹

¹Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Canada.

Scientific reports

|June 21, 2023

概括

阻断免疫检查点蛋白编程细胞死亡-1 (PD-1) 和淋巴细胞激活基因-3 (LAG-3) 可以增强不变的自然杀手T (iNKT) 细胞功能. 对PD-1和LAG-3的双重阻断显示出对抗免疫衰竭的前景.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

不变的自然杀手T (iNKT) 细胞对于免疫反应至关重要,但在癌症和艾滋病毒等慢性疾病期间可能会耗尽.
在iNKT细胞中,免疫衰竭的特征是免疫检查点蛋白的功能障碍和增加表达,包括PD-1和LAG-3.

研究的目的:

研究阻断PD-1和LAG-3以恢复iNKT细胞功能的潜力.
评估抗PD-1和抗LAG-3抗体疗法在增强iNKT细胞增殖和干扰素- (IFN-γ) 生产方面的疗效.

主要方法:

来自健康捐赠者的外周血液单核细胞用α-galactosylceramide进行了刺激.
流细胞计用于测量iNKT细胞上的LAG-3和PD-1表达.
一项为期10天的阻断试验评估了抗PD-1和抗LAG-3抗体对iNKT细胞增殖和IFN-γ产生的影响.

主要成果:

在iNKT细胞上的LAG-3和PD-1表达在刺激后的第四天达到峰值.
与对照组相比,抗PD-1和双抗PD-1/抗LAG-3阻塞都显著增加了iNKT细胞的增殖.
双阻塞策略在增强iNKT细胞增殖方面表现出卓越的有效性.

结论:

PD-1和LAG-3作为可行的免疫治疗点,用于增强人类iNKT细胞功能.
针对这些免疫检查点提供了一种潜在的策略,以克服iNKT细胞中的免疫疲劳.
双重阻断PD-1和LAG-3为改善iNKT细胞介导免疫提供了一个有希望的方法.

更多相关视频

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Published on: February 5, 2020

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Published on: February 15, 2021

相关概念视频

Cells of the Innate Immune Response

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...