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人母奶的巴氏消化会影响EVs的miRNA载荷,降低其免疫调节活性

Monica F Torrez Lamberti¹, Leslie A Parker², Claudio F Gonzalez¹

¹Department of Microbiology and Cell Science, Genetics Institute, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, USA.

Scientific reports

|June 21, 2023

概括

母乳消毒改变了细胞外囊泡 (EVs),降低了它们调节免疫反应的能力. 这影响了加工牛奶的生物活性潜力,突出了优化加工方法的需要.

科学领域:

新生儿营养新生儿营养
免疫学免疫学免疫学
生物技术是生物技术.

背景情况:

母乳 (MOM) 含有细胞外囊泡 (EVs),对婴儿免疫力至关重要.
消毒是一种确保牛奶安全的常用方法,但它对EV等生物活性成分的影响尚未完全理解.

研究的目的:

调查巴氏杀菌对MOM衍生的EVs的miRNA含量的影响.
评估巴氏杀菌如何影响MOM-EVs在先天免疫细胞上的免疫调节功能.

主要方法:

使用miRNA测序对本地MOM EVs (MOMEV) 和巴氏化MOM EVs (PMOMEV) 的miRNA资料进行比较分析.
使用Caco2肠道细胞和THP-1巨细胞进行体外分析,以评估MOMEV和PMOMEV的免疫调节作用.

主要成果:

消毒没有改变EV大小或粒子数,但显著改变了CD63标记物表达和miRNA配置文件.
在MOMEV和PMOMEV之间确定了33个差异丰富的miRNA.
与MOMEV相比,PMOMEV的IL-8分泌在Caco2细胞中的能力降低,IL-10和RNA感应基因在THP-1巨细胞中的诱导能力降低.

结论:

杀菌破坏了MOM-EVs的完整性和功能性免疫调节能力.
消毒后EV miRNA含量的变化与生物活性降低相关.
结果表明,优化牛奶加工的潜在生物标志物可以保护EV介导的生物活性.

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