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在小鼠早期怀孕期间,Notch信号通路成员的表达

Nuray Acar¹, Hakan Soylu², Sema Avci³

¹School of Medicine, Department of Histology and Embryology, Akdeniz University, Antalya, Turkey. nurayacar@akdeniz.edu.tr.

Journal of molecular histology

|June 21, 2023

概括

包括Hes7在内的痕信号对于怀孕早期和小鼠子宫内膜受容性至关重要. 这项研究详细介绍了围植过程中关键的Notch路径组件的表达模式.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
细胞信号传递细胞信号传递

背景情况:

健康的植入需要了解早期怀孕机制.
痕信号与生殖过程有关.
在周植入子宫中调查Notch通路组件至关重要.

研究的目的:

为了研究Notch 3,Notch 4,Rbp-J,Hes1,Hes7,Hey2,HeyL和Fbw7的表达和定位.
为了阐明Notch信号在小鼠子宫周植入期中的作用.
了解Notch信号对子宫内膜受容性的影响.

主要方法:

在不同的妊娠日 (D1,D4,D5,D6,D8) 中使用了Balb/C小鼠.
使用芝加哥蓝色染料识别了植入点.
雇佣了免疫组织化学 (IHC),西部涂抹 (WB) 和定量逆转录聚合酶链反应 (QRT-PCR).

主要成果:

排列3在第4天表现出强烈的积极表达.
在高孕激素 (P4) 水平 (4-8天) 中,4号位的表达很高.
4日Hes1水平最低;Hes7蛋白表达从D1增加到D8. 2在第1天和第4天达到峰值. 的局部化到腺体的果. 在第1天和第4天,Fbxw7的表达高.

结论:

痕信号在调节子宫内膜受容性方面发挥着重要作用.
观察到的Hes7表达模式是新的发现.
对Notch路径组件的进一步研究是有必要的,以了解植入.

关键词:

这是决定性的决定.鼠标鼠标是一个鼠标.口信号传输是没有的.怀孕怀孕怀孕怀孕在子宫,子宫.

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