大肠杆菌的新陈代谢工程用于高水平的紫丁的生产
Dong Xinrui1,2, Liu Bo3, Bao Yihong4,5
1College of Forestry, Northeast Forestry University, No. 26 Hexing Road, Harbin, 150040, Heilongjiang Province, People's Republic of China.
Microbial cell factories
|June 21, 2023
概括
经过代谢工程的大肠杆菌现在可以有效地产生紫素,这是一个有价值的植物. 这一突破使得这种重要的植物化合物的商业规模微生物生产成为可能.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 胡卜素生物合成 胡卜素生物合成
背景情况:
- 桑托菲尔,像紫素一样,是植物和藻类中重要的胡卜素,用于光采集和光保护.
- 维奥拉丁具有抗氧化特性和黄色色素,在医学,食品和化品方面具有潜力.
- 目前,由于异质宿主中的生物合成途径效率低下,商业规模的微生物生产紫素是有限的.
研究的目的:
- 开发一种代谢工程微生物平台,用于高效的紫素生产.
- 为了克服异质紫素生物合成途径的局限性.
- 建立一个可扩展的商业violaxanthin制造方法.
主要方法:
- 在Escherichia coli中对胡卜素生物合成底盘的系统优化.
- 改善关键紫素生物合成酶 (crtY,crtZ,ZEP) 的功能表达.
- 利用促进器库,低拷贝数等离子体和电子运输链共同表达用于应变工程.
主要成果:
- 经过工程设计的大肠杆菌菌株DZ12,在没有积累中间体的情况下产生了芝丁.
- 优化ZEP表达和其他因素导致了高产量的紫素生产.
- 达到约25.28±3.94毫克/g干细胞重量的紫素产量,并减少了副产品.
结论:
- 成功开发了一种高效的代谢工程大肠杆菌菌株,用于生产高产的紫素.
- 这项研究提出了第一个微生物平台,用于潜在的商业生产紫素.
- 工程菌株提供了一个可扩展的解决方案,用于制造这种有价值的桑托菲尔.
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