乙酸盐合成酶 (ALS) 中的突变会影响破碎 (Sorghum bicolor) 对抑制ALS的除草剂的反应
Ismail M Dweikat1, Malleswari Gelli2, Mark Bernards3
1Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA. Idweikat2@unl.edu.
Hereditas
|June 21, 2023
概括
耐除草剂的破碎甘由于广泛使用抑制乙酸盐合成酶 (ALS) 的除草剂而发展. 研究人员发现了新的破碎生物型,具有对ALS抑制除草剂的耐药性,有助于开发耐受作物.
科学领域:
- 农业学是一种农业学.
- 植物科学 植物科学
- 杂草科学杂草科学 杂草科学
背景情况:
- 碎 (Sorghum bicolor) 是北美农业的一个有问题的杂草.
- 广泛使用抑制乙酸盐合成酶 (ALS) 的除草剂导致了抗除草剂破碎的进化.
研究的目的:
- 为了识别和表征抗抗ALS抑制除草剂的破碎生物型.
- 为了研究破碎中抗除草药耐药性的遗传基础.
主要方法:
- 从有史以来硫应用的田间收集碎石甘种子.
- 进行温室评估和生物测试以评估除草剂反应.
- 测序了ALS基因以识别产生抗性的突变.
主要成果:
- 确定了三种破碎生物型 (P2-205,P8-30,P9-102),它们对抑制ALS的除草剂具有抗性.
- 生物型P8-30显示了一种独特的氨基酸替代 (Trp574到Leu).
- 在耐药生物型的ALS基因中检测到七个核酸替代.
结论:
- 鉴定的突变赋予了对ALS抑制除草剂的耐药性.
- 这一发现将有助于开发耐受于基于ALS的除草剂的 Sorghum 品种.
- 为农提供了新的杂草管理选择.
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