植物 Xanthomonas campestris pv. 的功能和结构特征 坎佩斯特里斯核心效应剂XopP揭示了一种新的激酶活性
Konstantinos Kotsaridis1,2, Vassiliki A Michalopoulou1,2, Dimitra Tsakiri1
1Department of Biology, University of Crete, Heraklion, 714 09, Crete, Greece.
The Plant journal : for cell and molecular biology
|June 22, 2023
概括
细菌效应剂XopP作为一种酶,酸化植物蛋白AtExo70B1并阻断其由宿主酶CPK5的酸化,从而逃避植物免疫力.
科学领域:
- 植物病原体相互作用
- 分子植物病理学 分子植物病理学
- 细胞机制 细胞机制
背景情况:
- Exo70B1是一种关键的外囊成分,参与植物免疫反应.
- 细菌效应剂XopP破坏了AtExo70B1的功能,但机制尚不清楚.
- 植物依赖的蛋白激酶5 (AtCPK5) 酸化物 AtExo70B1.1.
研究的目的:
- 为了阐明Xanthomonas campestris pv.的体内功能. 坎佩斯特里斯XopP (XccXopP) 在阿拉比多普西斯塔利亚纳Exo70B1 (AtExo70B1) 上.
- 描述XccXopP和AtExo70B1.1.之间的相互作用.
- 确定XccXopP是否具有激酶活性及其对AtExo70B1酸化的影响.
主要方法:
- 生物物理,生物化学和分子分析.
- 使用AlphaFold和DALI进行结构和功能预测.
- 对表达XccXopP的转基因Arabidopsis的分析.
主要成果:
- XccXopP与AtExo70B1形成稳定的相互作用,即使在高温下也是如此.
- 在多个位点 (Ser107,Ser111,Ser248,Thr309,Thr364) 的XccXopP酸化物AtExo70B1.
- 通过AtCPK5.5,XccXopP抑制了AtExo70B1的酸化.
结论:
- XccXopP作为一种针对AtExo70B1.1.的新型氨酸/氨酸激酶而起作用.
- XccXopP的激酶活性和AtCPK5酸化的抑制有助于绕过宿主免疫.
- 这项研究揭示了植物病原体相互作用中效应体介导的新毒性机制.
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