相关实验视频

Updated: Jul 26, 2025

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

基因组和表观遗传的变化驱动Rhabdomyosarcoma异常的骨肌肉分化

Silvia Pomella^1,2, Sara G Danielli³, Rita Alaggio⁴

¹Department of Hematology/Oncology, Cell and Gene Therapy, Bambino Gesù Children's Hospital, IRCCS Istituto Ospedale Pediatrico Bambino Gesu, Viale San Paolo 15, 00146 Rome, Italy.

|June 22, 2023

概括

轮骨髓肉瘤 (RMS) 涉及由肌源性调节因子 (MRFs) 调节的异常骨肌肉发育. 了解这些干扰提供了针对异常差异化的新治疗策略.

科学领域:

在瘤学瘤学.
发展生物学发展生物学
分子生物学分子生物学

背景情况:

狂肌肉瘤 (RMS) 是一种由异常的骨肌肉分化引起的儿科软组织肉瘤.
肌源性调节因子 (MRF) 控制正常的肌肉发育,但它们的失调有助于RMS瘤发生.
涉及超级增强剂的核心调节电路 (CRC) 在RMS中保持异常分化和瘤基因表达.

研究的目的:

审查RMS中异常肌肉分化背后的遗传和表观遗传机制.
探索这些机制如何促进RMS恶性病变和表型多样性.
基于针对异常分化途径来确定治疗机会.

主要方法:

审查关于骨肌肉发育,RMS和转录调节的现有文献.
对遗传和表观遗传因素的分析,包括MRF和CRC.
对单细胞研究的讨论,揭示了RMS中等级细胞子集.

主要成果:

CRCs在调节MRF表达和维持PF+ RMS中的PAX3::FOXO1融合癌基因中发挥着核心作用.
在RMS中异常肌肉分化涉及放松MRF活动和相互连接的CRC.
在RMS中分层组织的细胞子集重复了发育神经发生和驱动恶性瘤.

结论:

了解RMS异常肌肉分化的遗传和表观遗传框架对于阐明恶性瘤机制至关重要.
针对MEK/ERK信号或表观遗传机制等途径提供了潜在的治疗策略.
利用异常肌肉发育的原因,可以通过诱导终端分化来有效治疗RMS.

关键词:

不同化的差异化差异化.表观遗传学是指表观遗传学.基因组学就是基因组学.拉布多米索科马 (rhabdomyosarcoma) 是一种肌肉癌.这是骨肌肉的骨架肌肉.

更多相关视频

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

Generation of Myospheres From hESCs by Epigenetic Reprogramming

Generation of Myospheres From hESCs by Epigenetic Reprogramming

Published on: June 21, 2014

相关概念视频

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Satellite Stem Cells and Muscular Dystrophy

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...

Formation of Muscle Fibers from Myoblasts

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...

Genomic Imprinting and Inheritance

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...