在意大利的麦草中,外染色体DNA介导的草甘耐药性
Dal-Hoe Koo1, Yoonha Ju1, Karthik Putta2
1Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State University, Manhattan, KS, USA.
Pest management science
|June 22, 2023
概括
意大利麦草的草甘耐药性是由含有放大5-enolpyruvylshikimate-3-phosphate合成酶 (EPSPS) 基因的染色体外DNA介导的. 这种额外染色体DNA,大约400kb,通过基因放大赋予耐药性.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 杂草科学 杂草科学 杂草科学
背景情况:
- 意大利麦草的草甘耐药性与5-enolpyruvylshikimate-3-phosphate合成酶 (EPSPS) 基因的放大有关.
- 一个特定的意大利麦草种群表现出高水平的耐药性,原因是大约有70个EPSPS基因拷贝.
研究的目的:
- 为了物理地图放大EPSPS基因拷贝在抗草甘的意大利麦草.
- 阐明EPSPS基因放大赋予草甘耐药性的机制.
主要方法:
- 光在位杂交 (FISH) 用于对染色体上的EPSPS基因副本进行物理映射.
- 脉冲场凝电泳 (PFGE) 随后使用DNA涂抹来分析DNA碎片.
主要成果:
- 鱼类分析揭示了EPSPS信号在耐药植物的基相染色体上的随机分布,与易受植物不同.
- 通过PFGE和DNA涂抹,在耐药DNA中发现了一个突出的EPSPS杂交信号,约为400 kb,在敏感DNA中不存在.
结论:
- 该研究报告说,意大利的瑞草具有染色体外DNA介导的草甘耐药性.
- 放大后的EPSPS基因很可能位于染色体外的DNA元素上,大约400kb大小.
- 这种机制与其他植物物种中发现的染色体外DNA复制品相似.
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