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在小鼠精子生成中,Mrhl lncRNA的分子功能

Bhavana Kayyar^1,2, Shubhangini Kataruka^1,3, Vijay Suresh Akhade¹

¹Molecular Biology and Genetics Unit, JNCASR, Jakkur, Bangalore, India.

Reproduction (Cambridge, England)

|June 22, 2023

概括

Mrhl lncRNA控制了小鼠精子细胞中的Wnt信号传递和Sox8表达,促进了介质进入. 这种长长的非编码RNA通过循环调节染色质动态,影响精子生成.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子遗传学分子遗传学

背景情况:

精子生成涉及精子干细胞的受调的增殖和介质分裂.
长非编码RNAs (lncRNAs) 对于细胞分化和发育至关重要.
Mrhl lncRNA是一种单异构的 lncRNA,在小鼠丸中表达,并定位到核中,与染色素结合.

研究的目的:

为了研究Mrhl lncRNA在小鼠精子生成中的作用.
阐明Mrhl lncRNA调节Wnt信号和介质承诺的机制.
为了识别由Mrhl lncRNA调节的基因.

主要方法:

在小鼠丸和GC-1 spg细胞中分析Mrhl lncRNA表达.
由Mrhl lncRNA结合的基因组位置的映射.
研究Mrhl lncRNA在Wnt信号通路和Sox8基因调节中的作用.
染色体免疫沉 (ChIP) 试验用于研究蛋白质-DNA相互作用.

主要成果:

Mrhl lncRNA调节了小鼠精子细胞中的Wnt信号通路.
Wnt的激活降低了Mrhl lncRNA的调节,导致中介基因的激活和干细胞标记物的降低.
Mrhl lncRNA与37个基因进行物理关联,包括Sox8,通过促进体相互作用和涉及CTCF和YY1.1的染色体循环来调节其表达.
Mrhl lncRNA通过染色体循环介导Sox8促进剂/增强剂相互作用的染色体动力学.

结论:

Mrhl lncRNA在调节Wnt信号和Sox8表达方面发挥着至关重要的作用,从而控制小鼠精子生成中的介质进入.
Mrhl lncRNA的功能包括通过与目标基因和建筑蛋白质的物理关联来对染色质动态的表观遗传调节.
这项研究确定Mrhl lncRNA是精子分化和介质启动的关键调节者.

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