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Updated: Jun 21, 2026

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels
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Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

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通过氨酸策略,提高基于的人工甘氨酸酶的催化能力

Lijun Yang1, Yi Tian1, Yutong Wang1

  • 1School of Chemical Engineering and Technology, State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300350, P. R. China. mwang@tju.edu.cn.

Journal of materials chemistry. B
|June 22, 2023
PubMed
概括

研究人员使用氨酸开发了一种基于的人工甘氨酸酶. 这一策略增强了催化活性和稳定性,为类似酶的催化剂提供了一个新的平台.

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Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...