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在成人和胎儿组织中对水牛WNT10B基因的序列表征和比较表达特征

Deepu Verma^1,2, Manmohan Singh Chauhan¹, Shailendra Kumar Mishra²

¹ICAR-National Dairy Research Institute, Karnal, Haryana, India.

Animal biotechnology

|June 22, 2023

概括

无翼类型MMTV集成位点家族成员10B (WNT10B) 基因显示牛和水牛之间的遗传变异. 在胎儿和成年水牛组织之间,WNT10B基因表达有显著差异,这表明了更广泛的调节作用.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
进行比较基因组学.

背景情况:

无翼型MMTV整合部位家族成员10B (WNT10B) 基因在发育和疾病中至关重要.
了解像水牛这样的牲畜中的WNT10B,可以了解哺乳动物基因进化和功能.

研究的目的:

在印度水牛中进行WNT10B基因的序列表征.
分析牛和水牛WNT10B.B.之间的遗传变异.
为了研究WNT10B在胎儿和成年水牛中的组织特异性表达模式.

主要方法:

用DNA测序来表征WNT10B基因开放阅读框架.
牛和水牛之间的比较序列分析.
杂动物物种的遗传学分析.
实时定量PCR (RT-qPCR) 用于对各种胎儿和成人组织的基因表达分析.

主要成果:

水牛WNT10B基因有一个1176核酸开放的读取框架,编码一个391氨基酸蛋白质.
在牛和水牛WNT10B之间确定了19种核酸变异,导致6种氨基酸变化.
WNT10B在胎儿的小肠和肝脏以及成人的卵巢,丸,大脑,脏,小肠和肝脏中表达很高.
在胎儿和成人组织之间观察到WNT10B表达的显著差异,包括大脑,小肠,丸,脏,心脏,小肠和卵巢.

结论:

在牛和水牛之间的WNT10B基因中存在适度的遗传变异.
成年水牛中WNT10B的组织特异性表达模式表明,除了脂肪生成和血液形成之外还有其作用.
WNT10B可能在水牛的各种调节途径中发挥重要作用.

关键词:

这是WNT10B.布法罗水牛是什么意思胎儿组织胎儿组织基因表达的基因表达方式测序的测序是指测序的测序.

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