评估使用葡萄糖和纤维素作为碳来源的细菌物种生物表面活性剂的生产
Resa Setia Adiandri1,2, Ronny Purwadi1,3, Hoerudin Hoerudin2
1Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Jalan Ganesha No. 10, Bandung, West Java, 40132, Indonesia.
Current microbiology
|June 22, 2023
概括
基纤维素材料为生物表面活性剂生产提供了具有成本效益的基材. 细菌物种成功地使用从这些丰富的资源中获得的葡萄糖和树脂糖生产了高质量的生物表面活性剂.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 红纤维素生物质是微生物过程的丰富,低成本的原料.
- 林氏纤维素的水解产生葡萄糖和西洛斯,这是微生物生长和代谢物生产的关键碳来源.
- 生物表面活性剂是具有各种工业应用的表面活性化合物,但它们的生产成本仍然是一个挑战.
研究的目的:
- 为了评估使用葡萄糖和树脂糖的细菌物种的生物表面活性剂的生产.
- 评估基纤维素衍生糖作为碳来源的潜力,以成本效益高的生物表面活性剂合成.
- 为了描述由选定的Bacillus分离物产生的生物表面活性剂的质量.
主要方法:
- 通过分子16S rRNA测序确定了六种Bacillus细菌分离物.
- 使用葡萄糖和树脂糖作为唯一的碳来源诱导了生物活性剂的生产.
- 测量了生产的生物表面活性剂的表面张力降低和乳化稳定性.
主要成果:
- 这项研究确定了六种细菌物种:B. subtilis ITBCC46,B. subtilis ITBCC40,B. subtilis ITBCC31,B. siamensis ITBCC36,B. xiamenensis ITBCC43,以及B. subtilis ITBCC30.这些细菌物种包括:
- 所有测试的Bacillus物种都在葡萄糖和西洛斯介质上表现出增长.
- 四个分离物 (B. subtilis ITBCC46,B. subtilis ITBCC40,B. subtilis ITBCC31和B. siamensis ITBCC36) 产生了生物表面活性剂,使表面张力降低到40mN/m以下.
- 生物表面活性剂的乳化稳定性超过50%.
结论:
- 细菌物种可以有效地利用从纤维纤维素基材料中获得的葡萄糖和亚糖来生产生物表面活性剂.
- 生产的生物表面活性剂符合关键的质量标准,包括显著的表面张力降低和高乳化稳定性.
- 这项研究突出了基纤维素生物质作为工业规模生物表面活性剂制造可持续和经济原料的潜力.
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