番茄miR156-向的SLSBP15通过调节激素动态和与GOBLET和BRANCHED1b相互作用来抑制发芽的分支
Carlos Hernán Barrera-Rojas1, Mateus Henrique Vicente1, Diego Armando Pinheiro Brito1
1Laboratory of Molecular Genetics of Plant Development, Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), University of São Paulo (USP), Piracicaba, São Paulo, CEP: 13418-900, Brazil.
Journal of experimental botany
|June 22, 2023
概括
该miR156/SlSBP15枢纽通过连接植物激素和遗传通路来控制番茄芽的分支. 调节SLSBP15提供了塑造工厂架构的潜力.
科学领域:
- 植物生物学 植物生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 保存的miR156/SPL/SBP监管中心在拍摄架构中的作用尚未完全理解.
- 植物激素和控制尾芽发育的遗传途径之间的相互联系需要阐明.
研究的目的:
- 研究 miR156/SlSBP15 调节节点在调节番茄芽分支中的功能.
- 为了确定分子机制连接植物激素和遗传途径在尾芽调节.
主要方法:
- 在番茄植物中,miR156和一个抗miR156的SLSBP15等位基因过度表达.
- 分析发芽分支表型,尾芽发育和植物激素水平 (IAA,ABA).
- 基因和分子分析,包括辅酶运输抑制和与SlBRC1b.的相互作用.
主要成果:
- miR156的过度表达导致了高芽分支;rSBP15的过度表达导致了停止分支.
- 在miR156过度表达的植物中,rsBP15等位基因部分挽救了高分支的表型.
- SlSBP15通过抑制辅酶运输和GOBLET活性来调节叶芽的外生长,并与SlBRC1b相互作用以控制ABA水平.
结论:
- miR156/SlSBP15枢纽作为植物激素信号和控制番茄芽架构的遗传通路的关键集成者.
- SlSBP15在调节尾芽发育和外生长方面发挥着至关重要的作用.
- 准SLSBP15为操纵番茄植物架构提供了一个潜在的策略.
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