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在利比亚患者的慢性牙周炎中查17F介质素基因多态和八种下病原体

Eshraq Alsherif^1,2, Inas Alhudiri³, Mouna ElJilani³

¹Department of Life Sciences, Division of Microbiology, School of Basic Sciences, Libyan Academy, Tripoli, Libya.

The Libyan journal of medicine

|June 22, 2023

概括

慢性牙周炎 (CP) 与特定的牙下病原体和遗传因素有关. 这项研究发现P. intermedia是利比亚人的关键病原体,并确定了一种可能与CP严重程度相关的新型IL-17F基因变异.

科学领域:

牙周病学牙周病学
遗传学遗传学是一个
微生物学微生物学

背景情况:

慢性牙周炎 (CP) 是一种由病原体,遗传和环境因素影响的炎症状况.
干白素17 (IL-17) 细胞因子与CP病原发生有关.
了解下病原体和宿主基因之间的相互作用对于CP管理至关重要.

研究的目的:

在利比亚的皮肤病患者中,研究八种下病原体和IL-17F基因单核酸多态 (SNPs) 之间的关联.
在利比亚人口中识别与CP相关的特定细菌物种.
探索IL-17F基因变异在CP的发展和严重性中的作用.

主要方法:

一项涉及100名年龄在25-65岁之间的个体的病例控制研究.
多重PCR使用16SrRNA开头检测从纸质点样品中的子牙病原体.
桑格测序用于从口腔拭片DNA中识别IL-17F基因多态.

主要成果:

七种下牙病原体与CP具有高度显著的关联 (p<0.0001).
在一些病例中,发现了 *P.intermedia* (100%), *T.forsythia* (96%), *T.denticola* 和 *E.corrodens* (92%) 的高患病率.
一个新的IL-17F基因变异 (c.*34G>A) 与CP相关 (p=0.010),而IL-17F (rs763780) 显示没有关联 (p=0.334).

结论:

*P.intermedia*被确定为利比亚人口中CP的关键病原体.
一种IL-17F基因的新型变异可能导致慢性牙周炎的严重程度.
需要进一步的研究,以阐明这些病原体和遗传因素与CP联系的确切机制.

关键词:

它们的IL-17F是IL-17F.慢性牙周炎慢性牙周炎这是一个新的SNP.多形态主义的多态主义.亚牙病原体的病原体

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