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用细胞大小的脂质体评估阻抗流细胞计的准确性

Huichao Chai¹, Yongxiang Feng¹, Junwen Zhu¹

¹State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing 100084, P. R. China.

|June 22, 2023

概括

这项研究引入了一种使用脂质体验证阻抗流细胞计 (IFC) 中单细胞电模型的新方法. 这些发现为改进IFC系统设计和加强单细胞电气性能测量提供了关键指导方针.

科学领域:

生物物理学的生物物理.
微流体学微流体学
细胞电生理学细胞电生理学

背景情况:

单细胞电气特性作为疾病和免疫的重要无标签生物标志物.
阻抗流细胞计 (IFC) 是一种高通量方法,用于表征这些特性.
由于缺乏可靠的基准粒子,IFC的球形单电气模型的准确性仍未得到验证.

研究的目的:

开发和验证一种方法来评估IFC使用的单细胞电气模型的准确性.
建立可靠的,细胞大小的脂质体作为具有已知的电气参数的基准.
调查测量系统尺寸对IFC准确性的影响.

主要方法:

合成了细胞大小的单质脂质体,使用双乳液滴滴微流体.
雇佣IFC来测量这些脂质体的电性质.
实验评估了测量通道宽度 (w),高度 (h) 和电极间隙 (g) 对模型准确性的影响.

主要成果:

在电参数测量中,当传感区大小近似粒子大小时,相对误差低于10%.
确定通道高度 (h) 为最有影响力的参数,潜在误差高达~30%.
确定电极间隙 (g) 的误差略大于通道宽度 (w) 的误差.

结论:

开发的基于脂质体的方法为评估IFC模型提供了可靠的基准.
测量系统尺寸显著影响IFC准确性,而通道高度至关重要.
这项研究为优化IFC系统设计提供了必要的指导方针,以实现更准确的单细胞电气表征.

关键词:

准确度准确度准确度准确度准确度阻抗流量细胞计阻抗流量计.它们的内在属性是内在的.脂质体组是什么? 脂质体组是什么?一个单细胞分析.

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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