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基于MPT51和MPT64的抗原检测试验用于从尿样中诊断出肺外结核

Manisha Dass¹, Mohinder Kaur¹, Simran Aittan¹

¹Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.

Diagnostic microbiology and infectious disease

|June 22, 2023

概括

使用Mycobacterium结核病抗原MPT51和MPT64的基于尿液的新ELISA测试显示了诊断肺外结核 (EPTB) 的前景. 这种非侵入性测试可以帮助早期发现EPTB,与世卫组织的"结束结核病"战略保持一致.

科学领域:

传染性疾病传染性疾病
免疫学免疫学免疫学
诊断诊断诊断诊断

背景情况:

外肺结核 (EPTB) 诊断仍然具有挑战性,因为非特异性症状和有限的非侵入性测试.
世界卫生组织的"结束结核病"战略强调需要改善所有形式的结核病的诊断工具.
目前对EPTB的诊断方法通常涉及侵入性手术或耗时.

研究的目的:

开发和评估一种非侵入性,以尿为基础的酶相关免疫吸收试验 (ELISA),用于诊断肺外结核 (EPTB).
评估在尿样中准两种特定的Mycobacterium结核病抗原MPT51和MPT64的诊断性能.
确定开发的ELISA用于检测确切和可能的EPTB病例的灵敏度和特异性.

主要方法:

开发了一种基于尿液的ELISA,针对Mycobacterium结核病抗原MPT51和MPT64.
一组137名疑似EPTB患者 (包括肺结核,腹结核和结核性脑膜炎) 被分为"确定的"EPTB (n=10),"可能的"EPTB (n=77) 和"非EPTB" (n=50) 组.
使用来自"定义"EPTB和"非EPTB"组的ELISA结果生成接收器操作特征 (ROC) 曲线,以确定特异性和灵敏性的最佳切断值.

主要成果:

MPT51-ELISA获得了70%的灵敏度和86.3%的特异性,而MPT64-ELISA显示了90%的灵敏度和92%的特异性"定义"EPTB.
对于"可能"的EPTB,MPT51-ELISA的敏感度为32.5%,MPT64-ELISA的敏感度为30.8%.
结合两种ELISA,获得了"确定"EPTB的100%灵敏度和"可能"EPTB的41.6%灵敏度,具有80%的特异性.

结论:

针对MPT51和MPT64的基于尿液的ELISA显示出作为肺外结核病诊断的非侵入性查工具的巨大潜力.
综合试验对确定的EPTB具有高灵敏度,对可能的EPTB具有中度灵敏度,这表明它在识别需要进一步调查的患者方面具有实用性.
这种方法与改善结核病诊断的全球努力保持一致,并通过提供更容易获得的诊断选择来支持世卫组织的"结束结核病"战略.

关键词:

在腹部结核病.MPT51 MPT51 MPT51 在线播放 MPT64 MPT64 的使用方法多结核病是多结核病的一种疾病.结核性脑膜炎是一种基于尿液抗原的ELISA测试结果

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