基于反相/离子色谱混合模式静止相的超临界流体色谱,用于分离螺旋氧醇类化合物
Qing Fu1, Wenwen Dong1, Dandan Ge1
1Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
Journal of chromatography. A
|June 22, 2023
概括
使用新型混合模式静止相的超临界流体染色学 (SFC) 能够首次分离四种螺旋氧醇化物 (SOA). 这一进步为分析复杂的类化合物混合物提供了快速有效的方法.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 有机化学 有机化学
背景情况:
- 螺旋氧醇类化合物 (SOA) 是一种具有显著生物活性的复杂类自然产品.
- 在分析化学中,分离异构性SOA是一个相当大的挑战.
- 超临界流体染色学 (SFC) 由于其独特的溶剂特性,为具有挑战性的分离提供了一个有前途的技术.
研究的目的:
- 为了证明SFC在分离复杂的化物混合物的多功能性.
- 为了实现第一次分离四种特定的螺旋氧醇化物 (SOAs),包括异构体对.
- 阐明SFC中混合模式静止相的分离机制.
主要方法:
- 使用了两个反相/离子交换 (RP/IC) 混合模式静止相 (C8SAX和C8SCX).
- 研究了移动相组合 (例如,二甲基胺,酸,氨酸酸) 对类化合物保留的影响.
- 采用二氧化碳 (CO2) 作为主要移动相组件的SFC.
主要成果:
- 两个C8SAX和C8SCX阶段提供了同时分散和静电相互作用,对于SOA分离至关重要.
- 在C8SAX (EtOH + 10 mM酸格式) 和C8SCX (EtOH + 0.1%二甲) 上,优化移动相条件导致了明显的保留行为.
- 在5分钟内实现了基线分离的科里诺克赛因/异科里诺克赛因和林科菲林/异索林科菲林异构体.
结论:
- 具有混合模式静止相的SFC对于分离异构体螺旋氧醇类化合物非常有效.
- 了解移动相对保留机制的影响是优化SFC分离的关键.
- 开发的SFC方法可以快速有效地分析复杂的类化合物混合物.
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