从 pistachio 贝中发现心酸的异质性:一种用于结构特征的新方法
Giovanni Ventura1, Cosima Damiana Calvano1, Davide Blasi2
1Department of Chemistry, University of Bari Aldo Moro, via Orabona 4, 70126 Bari, Italy; Interdepartmental Research Center SMART, University of Bari Aldo Moro, via Orabona 4, 70126 Bari, Italy.
Food chemistry
|June 22, 2023
概括
研究人员使用一种新的环氧化和RPLC-ESI-MS/MS方法在豆中鉴定出酸 (AnAs) 的新型定位异构体. 这一发现使得进一步研究这些花生化合物的健康益处成为可能.
科学领域:
- 植物化学 植物化学
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
背景情况:
- 酸 (AnAs) 是 Anacardiaceae 家庭的关键二次代谢产物,特别是在 pistachios (Pistacia vera L.) 中.
- 在NA中双键的特殊安排显著影响其生物活性和潜在的健康益处.
- 了解ANA结构对于探索它们的应用至关重要.
研究的目的:
- 开发和验证一种新的分析方法,用于识别和量化果中的酸.
- 为了表征以前未被识别的无心酸的定位异构体.
- 通过阐明它们的结构多样性来研究ANA的潜在与健康相关的特性.
主要方法:
- 一个新的分析策略,结合RPLC分离和ESI-MS/MS检测,先进行环氧化反应.
- 该方法的应用是对化 pistachio 贝的绿色提取物.
- 方法验证用于定量 pistachio 乙醇提取物中的 AnAs.
主要成果:
- 在 pistachio shells 中确定具有13:0,15:0,和17: 1基链的占优势的 AnAs.
- 首次识别了AnA 15:1 (Δ8和Δ6) 和AnAs 17:1 (Δ10和Δ8) 的位置异构体.
- pistachio 乙醇提取物中 Ana 的量化,显示了超过 10 mg/100 g 生物质的度.
结论:
- 开发的环氧化-RPLC-ESI-MS/MS方法对于分析ANA及其异构体是有效的.
- 发现新的AnA定位异构体为研究其生物活性和健康益处开辟了新的途径.
- 豆是多样化的ANA的重要来源,需要进一步调查.
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