相关实验视频
Updated: Jul 26, 2025

11:11
Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
22.6K
爬行动物中的纳斯法-1:其作用和激素调节在壁的丸中
Krittika Dotania1, Mamta Tripathy1, Umesh Rai2
1Department of Zoology, University of Delhi, Delhi 110007, India.
General and comparative endocrinology
|June 22, 2023
概括
涅斯法-1通过促进壁丸中的精子生成和类固醇生成来调节爬行动物的繁殖. 这种是这种.
科学领域:
- 进行比较的内分泌学.
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
背景情况:
- 涅斯法-1是一种参与生殖的激素,在哺乳动物和鱼类中具有已知的作用.
- 它在爬行动物繁殖中的功能,特别是精子生成和类固醇生成,在很大程度上是未知的.
研究的目的:
- 研究墙壁 (Hemidactylus flaviviridis) 中 nucb2/nesfatin-1 的生殖阶段依赖表达和功能.
- 探索纳斯法-1在爬行动物中调节精子生成和类固醇生成中的作用.
主要方法:
- 在不同生殖阶段对nukb2/nesfatin-1表达的分析.
- 在实验室内用nesfatin-1化壁的丸,以评估对细胞增殖,分化,细胞亡标志物和类固醇生产的影响.
- 对丸核2/内沙丁-1mRNA表达的荷尔蒙调节 (FSH,DHT,E2) 的研究.
主要成果:
- 丸中的nukb2/nesfatin-1表达因生殖阶段而异,在活性阶段达到峰值.
- 纳斯法丁-1治疗刺激了精子生成标记物 (scf,c-kit,pcna),抑制了细胞灭绝 (caspase-3),并促进了抗细胞灭绝标记物 (bcl-2).
- 涅斯法丁-1增加了类固醇的急性调节蛋白 (星) 表达和的产生.
- 抑制了FSH,而DHT和E2刺激了纳斯法-1mRNA的表达.
结论:
- 涅斯法-1在壁的丸中,在调节精子生成和类固醇生成方面发挥着重要作用.
- 这项研究阐明了爬行动物丸中纳斯法丁-1表达的荷尔蒙控制.
- 提供了关于爬行动物的生殖内分泌学的新见解.
相关概念视频
Testosterone: Functions and Regulation
779
The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...
779
Testes: Histology
1.1K
A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...
The spermatogenic cells, responsible for producing sperm, are...
1.1K
Regulation of Hormone Secretion
3.6K
Regulation of hormone secretion is a finely tuned orchestration driven by various types of stimuli, encompassing neural, humoral, and hormonal signals. Environmental cues instigate neural stimuli, where action potentials traverse nerve fibers to reach their designated targets. An illustrative scenario is the body's response to stress, wherein the sympathetic nervous system releases epinephrine from the adrenal glands, inducing the well-known 'fight or flight' reaction.
Humoral...
Humoral...
3.6K
Hormonal Regulation
43.8K
Hormones regulate a significant portion of digestion through activation of the neuroendocrine system. The neuroendocrine system of digestion contains many different hormones all with multiple functions that are both, directly and indirectly, involved in digestion.
43.8K
Development of the Sexual Organs in the Embryo and Fetus
914
Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
914
Target Cell Response to Hormones
3.1K
Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
3.1K

