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缺少TRIC-B从第十四类型骨质发生不完善的骨质母细胞改变细胞粘附和线粒体功能 - 一个多omics研究研究

Milena Jovanovic¹, Apratim Mitra², Roberta Besio³

¹Section on Heritable Disorders of Bone and Extracellular Matrix, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States.

Matrix biology : journal of the International Society for Matrix Biology

|June 22, 2023

概括

在TMEM38B中发生的突变,导致Osteogenesis Imperfecta XIV型,损害骨质细胞功能. 缺少TRIC-B会破坏细胞粘附,增殖和线粒体健康,影响骨发育.

科学领域:

遗传学和分子生物学
细胞生物学细胞生物学
生物化学生化学

背景情况:

不完美的骨质发生 (Osteogenesis Imperfecta,简称OI) 是一组遗传性骨疾病.
第十四型OI是由TMEM38B的突变引起的,该突变编码TRIC-B通道.
以前的工作将TMEM38B的缺失与改变的ER流和原缺陷联系起来.

研究的目的:

研究TMEM38B在人类骨质母细胞分化和矿化中的作用.
阐明受TMEM38B缺陷在骨质母细胞中影响的细胞机制.

主要方法:

来自患者和小鼠的TMEM38B-null骨质母细胞的分析.
基因表达概况 (RNA-Seq) 和验证 (RT-PCR,西部斑).
评估细胞粘附,增殖,细胞循环和线粒体功能.

主要成果:

在TMEM38B-null骨质细胞中,矿化减少,骨质细胞标记基因表达发生改变.
降低细胞-细胞粘附通路的调节,包括间隙和紧密的接口.
骨质细胞增殖和细胞循环进展的受损.
线粒体延长,改变了融合/裂变动态,并增加了超氧化物生产.

结论:

TMEM38B/TRIC-B对于骨质母细胞的分化和功能至关重要.
TMEM38B 缺乏影响细胞对细胞的粘附,增殖和线粒体完整性.
这些发现突出了TMEM38B在骨发育和疾病中的新角色.

关键词:

细胞粘附细胞粘附线粒体分裂/融合骨质母细胞分化差异化骨质发生不完美症 (osteogenesis imperfecta) 是一个不完美的疾病.TMEM38B 在线播放这就是TRIC-B.

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