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肠道微生物群的组成对内毒素诱导的免疫反应或人类内毒素耐受性的发展没有预测价值

Niklas Bruse¹, Aron Jansen¹, Jelle Gerretsen¹

¹Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Department of Intensive Care Medicine, Geert Grooteplein Zuid 10, 6525 GA Nijmegen, the Netherlands; Radboud Center for Infectious Diseases (RCI), Radboud University Medical Center, Geert Grooteplein Zuid 10, 6525 GA Nijmegen, the Netherlands.

Microbes and infection

|June 22, 2023

概括

肠道微生物对人类免疫反应的影响有限. 虽然肠道细菌与免疫细胞细胞因子产生之间存在一些联系,但肠道微生物群并没有显著影响身体对炎症或耐受性的反应.

科学领域:

免疫学免疫学免疫学
微生物组研究微生物组研究
人类健康人类健康人类健康

背景情况:

肠道微生物组在调节人类免疫反应中的作用仍然在很大程度上是未知的.
研究肠道微生物群组成和免疫学表型之间的相互作用对于了解人类健康至关重要.

研究的目的:

确定肠道微生物群组成和健康志愿者的免疫表型之间的关系.
探索微生物群如何影响ex vivo和in vivo免疫反应.

主要方法:

用16S测序分析了108名健康志愿者,以分析他们的肠道微生物群.
使用ex vivo单细胞挑战模型来评估细胞因子反应.
使用脂聚糖 (LPS) 的体内挑战模型用于研究系统性炎症和内毒素耐受性.

主要成果:

在微生物群组成和ex vivo单细胞细胞因子反应之间发现了显著的关联,特别是IL-10的产生.
低差异 (0.3-4.8%) 由这些关联来解释,特定的属如Blautia, Bacteroides和Prevotella显示相关性.
在肠道微生物群组成和体内炎症参数之间没有显著的关系,也没有在LPS挑战后对内毒素耐受性的发展.

结论:

肠道微生物群的组成解释了ex vivo免疫细胞对病原性刺激的反应变异的有限部分.
在肠道微生物群和体内免疫反应或耐受性之间没有发现相关性.

关键词:

16S 测序的测序方法最后毒素 (Endotoxin) 是一种内毒素.我们的肠道微生物组.脂聚糖类糖化物脂聚糖类糖化物个性化医疗是个性化的医疗.宽容是一种宽容.

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