在MDCK细胞中,Tl (I) 和Tl (III) 诱导网膜应激
Emanuel Morel Gómez1, Cecilia I Casali2, María Del Carmen Fernández2
1Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Ciencias Biológicas, Cátedra de Biología Celular y Molecular, Buenos Aires, Argentina.
Environmental toxicology and pharmacology
|June 22, 2023
概括
暴露会阻止MDCK细胞的增殖,但保持活力. 它会诱导细胞内网膜应激和亡,这表明增殖障碍的机制.
科学领域:
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
背景情况:
- 是一种有毒的重金属.
- 了解的细胞作用对于毒理学和细胞生物学至关重要.
研究的目的:
- 研究 (I) 和 (III) 对马丁-达比犬 (MDCK) 细胞活力和增殖的影响.
- 阐明毒性背后的细胞机制,包括自,内质网膜 (ER) 应激和亡.
主要方法:
- 增殖的MDCK细胞暴露于.
- 细胞活力和增殖的评估.
- 对自标记物的分析 (SQSTM-1,LC3β,贝克林-1).
- 测量ER压力标志物 (ATF-6,IRE-1,XBP-1) 的测量.
- 细胞形态学的超结构分析.
- 对特定蛋白质表达的评估 (PHB,β-tubulin).
主要成果:
- 塔抑制了细胞增殖,同时保持了高细胞活力 (>95%).
- 自标志物发生了变化,48小时后SQSTM-1和贝克林-1表达增加.
- 在24小时内观察到显著的ER压力,由增加的ATF-6和IRE-1和XBP-1拼接表明.
- 超结构异常,包括ER扩大和细胞质真空化,在48小时后被发现.
- 增加的PHB和降低的β-tubulin表达表明潜在的帕帕托斯诱导.
结论:
- 暴露会在MDCK细胞中诱导内等质网膜应激.
- 可能会促进亡,导致增殖障碍.
- 这些发现提供了关于的细胞毒性机制的见解.
相关概念视频
01:17Role of ER in the Secretory Pathway
5.5K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
5.5K
01:31Regulation of the Unfolded Protein Response
2.5K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
01:23Stringent Response in E. coli
32
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
32
01:26Insertion of Single-pass Transmembrane Proteins in the RER
6.9K
Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
6.9K