母性NAT10在小鼠中调节卵细胞介质细胞循环的进展和成熟
Xue Jiang1, Yu Cheng2, Yuzhang Zhu3
1Reproductive and Genetic Hospital, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), 230001, Hefei, Anhui, China.
Nature communications
|June 22, 2023
概括
母性N-乙转移酶10 (Nat10) 对于小鼠卵细胞发育至关重要. 它调节mRNA降解,确保适当的介质进展和成熟.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 卵细胞成熟需要在转录和转录后的水平上精确调节介质细胞周期进展.
- 控制哺乳动物卵细胞中这些复杂过程的特定因素尚未完全理解.
研究的目的:
- 研究N-乙转移酶10 (Nat10) 在小鼠卵细胞发育中的作用.
- 阐明Nat10影响卵细胞成熟的分子机制.
主要方法:
- 在小鼠卵细胞中对Nat10功能的遗传分析.
- 关于ac4C修饰的特征及其对mRNA多项 (A) 尾巴长度的影响.
- 开发和应用一种新的头发针适配器-多 (HA-PAT) 测量方法.
主要成果:
- 纳10对于介质前期I进展,卵细胞生长和成熟至关重要.
- Nat10通过控制mRNAs的poly (A) 尾部降解来调节母体的转录组.
- 在CCR4-NOT复合体转录上,Nat10的ac4C沉积是这一规则的关键.
- 开发的HA-PAT方法提供了提高的灵敏度,效率和成本效益,用于poly (A) 尾长度分析.
结论:
- 母亲的 Nat10 在卵细胞发育中起着不可或缺的作用.
- Nat10介导的ac4C修饰是 oogenesis 中的一个关键的转录后调节机制.
- 该HA-PAT试验为研究mRNA衰变动态提供了有价值的工具.
相关概念视频
02:07Oogenesis
63.9K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.9K
02:02Meiosis II
45.9K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
45.9K
03:09Meiosis I
40.9K
Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
40.9K
02:57Meiosis vs. Mitosis
57.2K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
57.2K