类似月光的蛋白质实际上是Pseudocercospora fijiensis中的细胞壁组成部分
Blondy Canto-Canché1, Yamily Yazmin Burgos-Canul2, Deysi Chi-Chuc2,3
1Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Colonia Chuburná de Hidalgo, Calle 43 No. 130 x 32 y 34, Mérida, A.C., Yucatán, C.P. 97205, México.
World journal of microbiology & biotechnology
|June 22, 2023
概括
研究人员在真菌病原体Pseudocercospora fijiensis中发现了新的非典型细胞壁蛋白,这是黑色西加托卡病的原因. 这些蛋白质与典型的GPI固蛋白质不同,可能代表了一类参与真菌毒性的新类.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 生物化学 生物化学
背景情况:
- 细胞壁对于保护和致病性至关重要,它含有多种不同的蛋白质.
- 菲律宾伪科斯波拉 (Pseudocercospora fijiensis) 是黑色西加托卡 (Sigatoka) 的原因,它对香和植物构成重大威胁.
- 定于GPI的蛋白质是抗真菌策略的潜在目标.
研究的目的:
- 从Pseudocercospora fijiensis中分离和描述细胞壁蛋白质.
- 识别可能参与真菌病原发生的新型蛋白质.
- 探索控制植物真菌病的新目标.
主要方法:
- 隔离和广泛的洗的真菌细胞壁.
- 使用HF-pyridine进行蛋白质分离.
- 通过SDS-PAGE,电流和蛋白质测序.
- 西方斑点和蛋白质局部化组织学分析.
主要成果:
- 七种蛋白质被确定在一个突出的SDS-PAGE波段从HF-皮里丁分数.
- 发现的这些蛋白质中没有一个是GPI固的.
- 发现了非典型的,可能是新的细胞壁蛋白质,这表明了未知的结合机制.
- 这些蛋白质被证实是真正的细胞壁组成部分,并在真菌病原体中保存.
结论:
- 菲律宾的 Pseudocercospora fijiensis 拥有独特的一类非典型的细胞壁蛋白.
- 这些蛋白质很可能参与真菌病原和毒性.
- 这些发现表明开发针对这些新型蛋白质的抗真菌剂的新途径.
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