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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jul 26, 2025

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
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使用picoMeRIP-seqq进行单细胞m6的体内映射.

Yanjiao Li1,2, Yunhao Wang3,4, Maria Vera-Rodriguez1,5

  • 1Department of Microbiology, Oslo University Hospital, Rikshospitalet, Oslo, Norway.

Nature biotechnology
|June 22, 2023
PubMed
概括

我们开发了像素尺度的m6ARNA免疫沉和测序 (picoMeRIP-seq) 来映射单细胞和稀缺样本中的RNA甲基化. 这种方法需要最小的RNA,克服了当前体内研究技术的局限性.

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 目前的N6-甲基氨酸 (m6A) 测绘技术需要大量的RNA量.
  • 现有的方法通常仅限于培养细胞系,限制了体内应用.
  • 在稀缺的生物样本中需要敏感的m6A检测.

研究的目的:

  • 开发一种高度灵敏的m6A映射方法.
  • 为了使单细胞和低输入生物样本中的m6A分析成为可能.
  • 为了促进m6A修饰的体内研究.

主要方法:

  • 优化样本回收和信号与噪声比,用于免疫沉.
  • 开发了像素尺度的m6ARNA免疫沉和测序 (picoMeRIP-seq).
  • 使用标准实验室设备,以确保可访问性.

主要成果:

  • 成功地在图形RNA水平上绘制了m6A.
  • 证明了该方法在多种A) RNA和胚胎干细胞的定位方面的有效性.
  • 将picoMeRIP-seq应用于单个斑马鱼的卵巢,小鼠卵细胞和胚胎.

结论:

  • picoMeRIP-seq显著降低了用于m6A映射的RNA输入要求.
  • 该方法允许在单细胞和罕见细胞群中进行体内m6A分析.
  • picoMeRIP-seq为研究不同生物环境中的m6A动态提供了有价值的工具.