聚氨基酸生物合成和优化热友性Geobacillus stearothermophilus菌株K4E3_SPR_NPP的生物合成和优化
Seema Prabhudev Rodge1, Kundalik Shivaji Shende2, Niranjan Prakashrao Patil3
1Department of Microbiology, MES, Abasaheb Garware College, Pune, Maharashtra, India.
Extremophiles : life under extreme conditions
|June 22, 2023
概括
热友细菌Geobacillus stearothermophilus有效地产生聚酸酸盐 (PHA),一种可生物降解的塑料替代品. 使用响应表面方法和低成本基质 (如乳制品废水) 的优化可以提高PHA产量.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
背景情况:
- 塑料污染是一个重大的环境挑战.
- 生物降解的聚合物,如聚酸酸 (PHA) 提供了一个可持续的替代品.
- 极度友好的微生物是有效生产PHA的有希望的候选者.
研究的目的:
- 为了研究热友细菌Geobacillus stearothermophilus菌株K4E3_SPR_NPP.的PHA合成能力.
- 使用各种碳和源优化PHA生产.
- 评估使用工业废水用于PHA合成的潜力.
主要方法:
- 使用苏丹黑B和尼罗河红色染色进行PHA生产选.
- 通过克罗酸试验量化PHA含量.
- 使用1H-NMR光谱学识别PHA组成.
- 使用Plackett-Burman设计和响应表面方法来优化PHA生产.
主要成果:
- 基奥巴西勒斯 (Geobacillus stearothermophilus) 菌株K4E3_SPR_NPP与葡萄糖一起显示出显著的PHA积累 (31%的PHA/DCW).
- 生产的PHA被确定为一个中链长度共聚物 (PHB-PHV-PHHx).
- 作为基质的乳糖和酸产生了分别45%和53%的PHA/DCW.
- 在优化条件下,PHA积累率为66.66% (0.32 g/l PHA).
- 使用乳制品行业的废水导致PHA积累45% (0.33g/lPHA).
结论:
- 地基菌 (Geobacillus stearothermophilus) 是一种可行的热爱PHA的生产者.
- 优化策略显著提高PHA产量.
- 低成本的工业基质,如乳制品废水,适合PHA生产.
- 热性PHA生产为可持续生物塑料提供了一个有前途的途径.
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