产生butanol的Clostridium sp.的主要基因组特征 在UCM В-7570菌株
Olena Tigunova1, Markiyan Samborskyy2, Viacheslav Bratishko3
1Institute of Food Biotechnology and Genomics of the NAS of Ukraine, 2a Baida Vyshnevetskyi str, Kyiv, 04123, Ukraine. Tigunova@ukr.net.
Journal of applied genetics
|June 22, 2023
概括
基因组测序发现了Clostridium sp. 的存在. 在UCM B-7570作为Clostridium pasteurianum. 在UCM B-7570作为Clostridium pasteurianum. 在UCM B-7570作为 这种细菌显示出对代谢工程的潜力,特别是在butanol发酵途径中.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物技术是生物技术.
背景情况:
- 测序技术的进步为细菌基因组和代谢途径提供了新的见解.
- 了解遗传组织对于开发用于工业应用的改良细菌菌株至关重要.
研究的目的:
- 为了测序和描述Clostridium sp.的基因组. 在UCM B-7570生产菌株.
- 分析参与butanol发酵的基因,并确定菌株的分类学分类.
主要方法:
- 全基因组测序和组装.
- 对已识别的基因和蛋白质序列的生物信息分析.
- 进行比较基因组学以确定菌株的相关性.
主要成果:
- 这是Clostridium sp.的基因组. UCM B-7570被组装为4.47Mbp,GC含量为29.7%,含有4262个基因.
- 鉴定了butanol发酵酶的基因,并发现它们是集群的.
- 蛋白质序列与Clostridium pasteurianum类型菌株的相似性最高.
结论:
- 克洛斯特里迪亚 sp. UCM B-7570被确定为Clostridium pasteurianum. 这种菌种的原始成分是菌.
- 这种菌株拥有有利于代谢工程的基因组,用于增强butanol生产.
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