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刺激拉曼组织学用于口腔状细胞癌的组织学评估

David Steybe^1,2,3, Philipp Poxleitner^4,5, Marc C Metzger⁴

¹Department of Oral and Maxillofacial Surgery, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany. david.steybe@uniklinik-freiburg.de.

Clinical oral investigations

|June 22, 2023

概括

刺激拉曼组织学 (SRH) 在口腔状细胞癌 (OSCC) 手术中准确区分瘤与非瘤组织. SRH显示了新鲜组织在不染色或切割的情况下进行手术内成像的潜力.

科学领域:

组织病理学组织病理学
手术瘤学手术瘤学
光学成像技术的成像

背景情况:

口腔状细胞癌 (OSCC) 的手术需要精确的组织边缘的手术内评估.
传统的冷截面分析用血素和乙素 (H&E) 染色是耗时的,需要组织加工.

研究的目的:

为了评估刺激拉曼组织学 (SRH) 的诊断准确性,与H&E染色的OSCC冷部分相比.
评估SRH在手术期间区分瘤与非瘤组织的能力,并对非瘤组织进行分类.

主要方法:

用SRH从8名OSCC患者的80个组织样本中生成数字组织病理图像.
用H&E染色的冷部分从相同的样本中获得,以便进行比较.
计算了SRH和H&E之间的一致性和准确性 (灵敏度,特异性,PPV,NPV,AUC).

主要成果:

在区分瘤与非瘤组织方面,SRH表现出高一致性 (kappa:0.880) 和精度 (AUC:0.954).
在检测OSCC时,SRH获得了100%的灵敏度和90.91%的特异性.
对于非新发性组织,SRH对正常的粘膜,肌肉和唾液腺具有很高的准确性.

结论:

在OSCC中,SRH是一种非常准确的方法来区分瘤和非瘤组织.
SRH提供了新鲜OSCC组织样本的快速,手术内分析的潜力,减少了传统染色和切割的需要.

关键词:

显微镜的使用方法口腔状细胞癌的癌症.病理学病理学病理学拉曼光谱法拉曼光谱法外科手术的边缘是什么

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