种类内的多样性Clostridium butyricum:泛基因组,系系,prophage,碳水化合物利用,以及抗生素耐药性
Zhangming Pei1,2, Yufei Liu1,2, Zhi Yi1,2
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
Journal of applied microbiology
|June 23, 2023
概括
作为一个有前途的益生菌,Clostridium butyricum显示出显著的遗传多样性和共同的生态. 它的基因组含有菌体,但CRISPR系统提供防御,菌株普遍利用关键碳水化合物并抵抗某些抗生素.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 益生菌 益生菌 益生菌
背景情况:
- 由于其健康益处,Clostridium butyricum是下一代益生菌的有希望的候选者.
- 对C.butyricum遗传和表型多样性的有限理解需要进行全面的菌株分析.
研究的目的:
- 评估Clostridium butyricum的基因组和表型多样性.
- 了解C.butyricum属性的遗传基础,包括碳水化合物利用率和抗生素耐药性.
主要方法:
- 隔离了53种C.butyricum菌株,并分析了25个公共基因组.
- 使用了平均核酸标识和遗传学分析.
- 研究了体元素和CRISPR-Cas系统.
主要成果:
- 识别了具有开放泛基因组和融合核心基因组的广泛基因组多样性.
- 在各种菌株中观察到纤维素,酸盐和可溶性粉的普遍利用.
- 已证明对氨基糖类抗生素的一般耐药性和CRISPR介导的prophage抑制.
结论:
- 克洛斯特里黄油表现出广泛的遗传变异和无处不在的prophages,与CRISPR提供防御.
- 特定的基因型与碳水化合物代谢和抗生素耐药性的观察到的表型相关.
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