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Expression and Purification of Virus-like Particles for Vaccination
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开发具有内置免疫刺激性质的病毒样颗粒,作为疫苗候选剂
Simon Collett1, Linda Earnest2, Julio Carrera Montoya2
1School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC, Australia.
Frontiers in microbiology
|June 23, 2023
概括
这项研究开发了针对SARS-CoV-2和登革热等包裹RNA病毒的新型病毒样粒子 (VLP) 疫苗候选人. 配合α-galactosylceramide辅助剂的配方有效地刺激了免疫反应,提供了一个有前途的新疫苗平台.
科学领域:
- 疫苗学 疫苗学 疫苗学
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 病毒样粒子 (VLP) 疫苗对一些病毒取得了成功,但面临着登革热和SARS-CoV-2等包裹病毒的挑战.
- 技术障碍包括了解保护性免疫力和大规模制造包装VLP疫苗.
- 开发针对迅速突变的RNA病毒 (如SARS-CoV-2和登革热) 的有效疫苗至关重要.
研究的目的:
- 开发和描述针对SARS-CoV-2和登革热病毒 (DENV) 的新型VLP疫苗候选者.
- 评估一种新型的疫苗配方,其中包含辅助剂α-galactosylceramide (α-GalCer).
- 评估这些VLP候选人的免疫性在体外和体内.
主要方法:
- 使用SARS-CoV-2和DENV的主要结构蛋白的VLP开发.
- 使用西方免疫斑块,酶免疫试验,电子显微镜和原子力显微镜进行表征.
- 在体外和体内免疫性研究评估T细胞和抗体反应.
主要成果:
- 显微镜证实了蛋白质的自我组装成对本地病毒真实的VLP.
- 包括α-GalCer辅助剂在体外刺激了高水平的自然杀手T (NKT) 细胞.
- 在 SARS-CoV-2,HCV 和 DENV VLP 的体内观察到显著的抗体和记忆 CD8+ T 细胞反应.
结论:
- 开发的VLP候选疫苗在结构上对本地病毒是真实的.
- α-GalCer辅助剂增强了先天性和适应性免疫反应.
- 这种新型VLP疫苗配方代表了对抗由包裹RNA病毒引起的感染的有希望的平台.
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