通过电荷密度调制产生的动态挫败电荷热点将球状蛋白质隔离到复杂的同体中.
Biplab K C1, Teruki Nii2, Takeshi Mori2,3
1Graduate School of Systems Life Sciences, Kyushu University 744 Moto-oka, Nishi-ku Fukuoka 819-0395 Japan.
Chemical science
|June 23, 2023
概括
研究人员开发了合成多联体来隔离球状蛋白质,模仿生物凝结物. 这种支架-客户端系统为人工器官和生命科学研究提供可调节的特性.
科学领域:
- 生物材料科学 生物材料科学
- 超分子化学 超分子化学
- 合成生物学 合成生物学
背景情况:
- 生物凝结物通过支架-客户端相互作用形成,对于细胞组织至关重要.
- 在合成系统中复制这些相互作用是理解生物过程和创造人工有机体的关键.
- 现有的合成支架往往缺乏在自然系统中发现的细微相互作用.
研究的目的:
- 开发一种简单的策略,将球状蛋白质隔离成基于合成聚的复杂协体.
- 用合成材料来回顾在生物凝结物中发现的支架-客户互动.
- 通过合理的设计来研究同性质的可调性.
主要方法:
- 合成了具有较低线性电荷密度 (σ) 的聚离子 (聚乙烯糖醇-b-聚酸) .
- 复杂的协体是使用减少的σ聚离子和 homo-poly-l-lysine (聚) 形成的.
- 测量了蛋白质测序效率,并使用光漂白后光恢复 (FRAP) 探测了相互作用.
主要成果:
- 复杂的协生物有效地隔离了各种球状蛋白质 (>80%的封装效率).
- 蛋白质结合主要是由静电相互作用驱动的,这取决于离子强度和蛋白质电荷.
- 协体内的动态挫败电荷被确定为与蛋白质的静电相互作用的起源.
- FRAP的测量表明,蛋白质相互作用改变了同体的动态,证明了可调的物理性质.
结论:
- 建立了一个合理的设计方法,用于合成协同的支架-客户端交互.
- 合成的协生物有效地模仿生物凝聚物功能,作为球状蛋白质的支架.
- 这项工作为开发人造器官和推进基本生命科学研究提供了基础.
相关概念视频
Globular Proteins
7.5K
In organisms, proteins are the most abundant macromolecules. They act as the building blocks of life and play various crucial roles in the body. Proteins can be broadly classified into two distinct subtypes based on their shape and solubilities: globular proteins and fibrous proteins.
Globular proteins serve many important physiological functions, such as acting as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be soluble in the aqueous...
Globular proteins serve many important physiological functions, such as acting as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be soluble in the aqueous...
7.5K
Cooperative Allosteric Transitions
7.9K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
7.9K
Complexation Equilibria: The Chelate Effect
569
In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...
569
Protein Complex Assembly
10.7K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.7K
Globular and Fibrous Proteins
43.9K
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
43.9K
Assembly of Signaling Complexes
5.8K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.8K


