层次性的共价有机框架-用于多酶联催化物的泡
Satyadip Paul1,2, Mani Gupta3, Kaushik Dey1,2
1Department of Chemical Sciences, Indian Institute of Science Education and Research Mohanpur Kolkata 741246 India.
Chemical science
|June 23, 2023
概括
通过使用二氧化碳泡合成了等级共价有机框架 (COF) 泡,增强了酶固定和活性. 这些COF泡能够有效地将碳甲基纤维素转化为葡萄糖,展示了先进生物催化剂的潜力.
科学领域:
- 材料科学 材料科学 材料科学
- 生物催化剂是一种生物催化剂.
- 纳米技术纳米技术
背景情况:
- 联有机框架 (COF) 为生物分子固定提供高孔径,但受到微孔的限制,阻碍了大型酶负载和基质扩散.
- 具有微,中,大孔的层次性多孔结构有利于酶催化,提高固定效率和反应动力学.
研究的目的:
- 开发一种具有增强孔隙性的层次化的COF泡矩阵,以实现高效的酶固定和生物催化.
- 为了研究在新型COF泡结构中的固定细胞分解酶 (β-葡萄糖酶,细胞氧化酶,内葡萄糖酶) 的性能.
主要方法:
- 合成了层次化的TpAzo COF-泡,使用现场CO2气体泡技术,在有序的2D COF纳米结构中创建宏孔.
- 将多个细胞分解酶 (BGL,CBH,EG) 固定在TpAzo泡基质上.
- 评估了基质水解的酶活性,在有机溶剂中的稳定性和运动参数 (Km,Vmax).
主要成果:
- 与传统的COF相比,等级化的TPAzoCOF泡表现出1.5至4倍更高的酶固定效率.
- 固定酶保持了高活性,BGL在有机溶剂和室温下表现出稳定性.
- 多酶固定的TpAzo泡成功催化了碳素甲基纤维素在10个循环中将其转化为葡萄糖的单并列转化.
结论:
- 层次化的COF泡结构有效地克服了微孔COF在酶固定和催化方面的局限性.
- 开发的TpAzo COF-泡是高效,可循环的多酶系统在协同生物催化剂中的一个有前途的平台.
- 这种方法为设计用于工业生物催化应用的先进酶固定材料开辟了新的途径.
相关概念视频
Catalytically Perfect Enzymes
4.0K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Most enzymes...
Most enzymes...
4.0K
Cofactors and Coenzymes
11.1K
Enzymes are proteins made of amino acids. The functional group of each constituent amino acid catalyzes a wide variety of chemical reactions via ionic interactions or acid-base reactions. However, amino acids cannot catalyze oxidation-reduction and group transfer reactions and need to be aided by non-protein components called cofactors. Cofactors are also referred to as the chemical teeth of an enzyme.
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
11.1K
Catalysis
27.1K
The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
27.1K
Cooperative Allosteric Transitions
7.9K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
7.9K
Introduction to Enzymes
18.8K
The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that...
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that...
18.8K
Enzyme Kinetics
97.3K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
97.3K


