Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Critical period plasticity enables credit assignment.

bioRxiv : the preprint server for biology·2026
Same author

Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

Journal of biomedical optics·2026
Same author

SAMJ: fast image annotation on ImageJ/Fiji via segment anything model.

Nature communications·2026
Same author

Kiss and spit metabolomics highlight the role of host purine metabolism during pathogen infection.

mSphere·2026
Same author

Search, organize, aggregate and share image data with BioFile Finder (BFF).

Nature methods·2026
Same author

Organoid Level Assessments of Human Primary and Metastatic Colorectal Cancer-Derived Organoids Predict Response to Chemotherapy and Chemoradiation.

Cancers·2026

相关实验视频

Updated: Jul 25, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

786

用单光子雪崩二极管阵列进行光板自光终身成像.

Kayvan Samimi1, Danielle E Desa1, Wei Lin2

  • 1Morgridge Institute for Research, Madison, Wisconsin, United States.

Journal of biomedical optics
|June 23, 2023
PubMed
概括

我们开发了一种新的光片光终身成像显微镜 (FLIM) 系统,用于使用尼古丁胺胺氨基二核酸 (酸盐) [NAD(P) H]监测细胞代谢. 这种先进的技术为实时3D细胞成像应用提供了显著的速度优势.

关键词:
自动光是一种自发光.光终身成像显微镜显微镜光片显微镜光片显微镜尼古丁胺胺腺因二核酸 (酸盐)一个光子雪崩二极管

更多相关视频

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

8.5K
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

25.3K

相关实验视频

Last Updated: Jul 25, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

786
Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

8.5K
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

25.3K

科学领域:

  • 生物物理学的生物物理.
  • 细胞的新陈代谢
  • 显微镜的使用方法

背景情况:

  • 光终身成像显微镜 (FLIM) 尼古丁胺氨酸二核酸 (酸盐) [NAD(P) H]对于研究活体3D系统中单细胞代谢至关重要.
  • 目前用于NAD的FLIM方法缺乏光板显微镜所提供的速度和光学切割能力,特别是用于快速成像活体3D样本.

研究的目的:

  • 设计,验证和演示NAD的概念验证灯片系统.
  • 为了实现更快,更有效的3D活细胞对代谢过程的成像.

主要方法:

  • 将单光子雪崩二极管摄像机集成到光板显微镜中.
  • 光学切割以最大限度地减少失焦的光贡献.
  • 使用光寿命标准和胰腺癌细胞中的代谢干扰研究进行验证.

主要成果:

  • 成功构建和验证了NAD(P) H光板FLIM系统.
  • 在癌细胞中代谢变化的10秒整合时间成像和斑马鱼中中性粒细胞的体内成像的演示.
  • 与激光扫描相比,对光板几何学来说,量化了显著的速度优势 (高达X倍),而对于NAD (P) H FLIM来说则是激光扫描.

结论:

  • 光板几何是可行的,对于NAD(P) H FLIM来说是非常有利的.
  • 这项技术对3D活细胞成像具有吸引力,包括监测免疫细胞代谢和生物体内的迁移.