柴油废气颗粒改变了熊蜂的肠道微生物组和基因表达Bombus terrestris
Dimitri Seidenath1, Alfons R Weig2, Andreas Mittereder3
1Animal Ecology I, Bayreuth Center of Ecology and Environmental Research (BayCEER) University of Bayreuth Bayreuth Germany.
Ecology and evolution
|June 23, 2023
概括
柴油废气颗粒 (DEP) 通过破坏它们的肠道细菌并引起压力反应来伤害大黄蜂. 这种口服暴露会影响昆虫的健康,可能会增加病原体的易感性并减少能源资源.
科学领域:
- 环境科学 环境科学
- 生态毒理学 生态毒理学
- 微生物学 微生物学
背景情况:
- 昆虫的减少对生态系统功能 (如授粉和害虫控制) 构成重大全球威胁.
- 人为污染,包括空气中的颗粒物,是昆虫减少的关键驱动因素,但其具体影响仍未得到充分研究.
- 虽然农药的影响是已知的,但其他污染物如柴油废气颗粒 (DEP) 对昆虫健康的影响需要进一步调查.
研究的目的:
- 研究柴油废气颗粒 (DEP) 和制动粉尘对大黄蜂 (Bombus terrestris) 的肠道微生物和基因表达的影响.
- 为了确定这些污染物的口腔或空气暴露是否会导致大黄蜂的显著生理变化.
主要方法:
- 在7天内,Bombus terrestris的工人通过口服或吸入暴露于低致命剂量的DEP和制动粉尘.
- 使用测序技术分析了肠道微生物组的组成.
- 用转录组分析评估基因表达的变化.
主要成果:
- 口服暴露于DEP显著改变了肠道微生物组结构,特别是减少了核心细菌Snodgrassella的丰度.
- 转录组分析显示,在口服DEP暴露后,与代谢和信号转导相关的基因表达发生了显著的变化,这表明了应激反应.
- 暴露于车粉尘或空气中的DEP并没有导致肠道微生物组或基因表达的显著变化.
结论:
- 口服暴露于柴油废气颗粒可以诱导肠道失调和熊蜂的一般应激反应.
- 这些影响可能会通过增加对病原体的易感性和耗尽能量储备来危害大黄蜂的健康.
- 这些发现强调了空气污染对昆虫种群的潜在风险,特别是在复杂的多压力环境中.
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