建立和表征一个不朽化的牛肠上皮细胞系
Sudan Meng1,2, Y Uexin Wang1, Shuai Wang1
1Henan International Joint Laboratory of Animal Welfare and Health Breeding, Henan University of Science and Technology, Luoyang 471023, China.
Journal of animal science
|June 23, 2023
概括
研究人员开发了一种不朽的牛肠上皮细胞系 (BIECs-21),用于研究肠道疾病. 这种稳定的细胞系模仿原生细胞,使得对牛肠道病原体感染的先进研究成为可能.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 主要牛肠上皮细胞 (PBIECs) 对于研究牛肠道疾病至关重要.
- 对PBIEC的挑战包括稳定培养和短寿命的困难,限制了研究应用.
研究的目的:
- 为了建立一个稳定的,不朽的细胞系,用于在体外研究牛肠表皮细胞病原体感染机制.
- 为了克服与初级细胞培养相关的局限性.
主要方法:
- PBIECs的孤立和文化.
- 使用编码人类端粒酶逆转录酶 (hTERT) 的pCI-neo-hTERT等离子体对PBIEC进行不朽化.
- 永生细胞系的表征 (BIECs-21) 结构和功能与PBIECs相似.
主要成果:
- 永久化的BIECs-21细胞系保留了PBIEC的结构和功能,表达了关键的上皮标记物 (细胞卡拉丁18,奥克卢丁,ZO-1,E-cadherin,肠酶).
- BIECs-21表现出正常细胞特征 (生长,型,血清依赖,接触抑制) 和转移的hTERT基因的稳定,高表达.
- 与PBIEC相比,细胞系表现出一致的短链脂肪酸运输和葡萄糖吸收,并且易受Neospora caninum感染.
结论:
- 永久化的BIECs-21细胞系为牛肠道研究提供了稳定且功能性的体外模型.
- 这种细胞系是研究牛肠表皮中的病原体感染机制的宝贵工具.
- BIECs-21克服了原始细胞的局限性,促进了牛胃肠病学的先进研究.
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