技术进步定义了从正常转移信号通路到原发性和转移性结直肠癌的信号通路
Robert G Ramsay1,2, Vicki Whitehall3,4, Michael P Flood1
1Sir Peter MacCallum Department of Oncology and Peter MacCallum Cancer Centre, The University of Melbourne, Parkville, Australia.
Growth factors (Chur, Switzerland)
|June 23, 2023
概括
有机体和瘤体模型通过模仿瘤及其微观环境,为研究结直肠癌 (CRC) 提供了新的途径. 这些患者衍生模型加速了化学疗法和免疫疗法等疗法的测试.
科学领域:
- 瘤学和癌症生物学
- 胃肠病学 胃肠病学
- 3D细胞培养和机体技术
背景情况:
- 肠道上皮质的有机和瘤传播提供了一个平台来比较细胞依赖.
- 这些3D模型总结了瘤基因表达和瘤微环境 (TME).
- 通过添加淋巴细胞和与癌症相关的纤维细胞等组件来操纵TME.
研究的目的:
- 探索有机体/瘤体模型在理解结肠直肠癌 (CRC) 生物学方面的实用性.
- 调查这些模型在快速治疗测试中的潜力.
- 为CRC研究利用有机体技术和单细胞RNA测序 (scRNAseq) 的进步.
主要方法:
- 正常和恶性肠道上皮质有机体/瘤体的传播.
- 纳入和操纵瘤微环境组件 (如淋巴细胞,纤维细胞).
- 单细胞RNA测序 (scRNAseq) 的应用用于详细的分子分析.
- 测试各种疗法的疗效,包括化疗,放射治疗和免疫治疗.
主要成果:
- 有机体/瘤体模型准确地反映了瘤基因表达和TME贡献.
- 治疗效果 (化疗,放射,免疫疗法) 可以在几周内评估.
- 这些模型提供患者特定的瘤化身,以获得个性化的治疗见解.
- 在有机体传播和scRNAseq方面的进步正在完善对结直肠癌亚型 (CMS) 的理解.
结论:
- 有机体/瘤体模型是研究结直肠癌 (CRC) 和其微观环境的强大工具.
- 这些来自患者的化身能够快速和个性化地进行治疗查.
- 新兴技术正在改变CRC亚型的分类和理解.
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