类花粉1通过控制大米中UDP-糖的恒常性来调节ROS触发的PCD和花粉壁的发展
Huiqiong Chen1,2, Shuqing Zhang1, Ruiqi Li1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangdong Laboratory for Lingnan Modern Agriculture College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
The Plant cell
|June 23, 2023
概括
米的生育能力由UDP-glucose 4-epimerase (UGE) 调节,这种酶对UDP-糖的恒温至关重要. 由于UDP-Glc的积累,Botryoid花粉1 (bp1) 突变中的UGE功能丧失导致异常的子发育.
科学领域:
- 植物生物学 植物生物学
- 生物化学 生物化学
- 分子遗传学 分子遗传学
背景情况:
- 尿素二酸盐 (UDP) - 糖是聚糖合成和潜在信号分子中的关键代谢物.
- 在大米 (Oryza sativa) 生育和其他发育中UDP-葡萄糖4-表皮酶 (UGE) 的作用尚不清楚.
研究的目的:
- 为了识别和描述负责状花粉1 (bp1) 突变的基因.
- 阐明UGE在调节UDP-糖平衡中的作用及其对大米和其他发展的影响.
主要方法:
- 在大米中的bp1突变体的遗传分析.
- 生物化学试验测量UDP-糖的水平.
- 对活性氧物种 (ROS) 积累和编程细胞死亡 (PCD) 的分析.
- 检查花粉壁 (内膜和外膜) 的形成.
主要成果:
- 这种bp1突变表型是由编码UGE的基因突变引起的.
- 失去BP1功能会导致大量的UDP-葡萄糖积累以及其他UDP-糖的不平衡.
- 升高的UDP-葡萄糖诱导NADPH氧化酶的表达,导致ROS过早积累和PCD.
- 由于UDP-葡萄糖积累而导致异常的质降解,导致粘性微胞和花粉壁形成的缺陷.
结论:
- BP1/UGE对于保持UDP-糖平衡至关重要,这对米的其他发展至关重要.
- UDP-葡萄糖作为一个信号分子,可以触发ROS积累和PCD.
- 在花粉发育过程中,UDP-糖代谢涉及到内部和外部形成.
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