小麦DOF转录因子TaSAD和WPBF与SPA合作调节谷蛋白基因表达
Marielle Merlino1, Jean-Charles Gaudin2, Mireille Dardevet1
1INRAE, Clermont Auvergne University, UMR GDEC, Clermont-Ferrand, France.
PloS one
|June 23, 2023
概括
研究人员发现了一种新的小麦转录因子TaSAD,它对调节谷物储存蛋白 (GSP) 合成至关重要. 这一发现提高了对小麦质量的理解,并为改善GSP表达提供了目标.
科学领域:
- 植物分子生物学 植物分子生物学
- 农业科学 农业科学
- 生物化学 生物化学
背景情况:
- 谷物储存蛋白 (GSP) 对小麦面粉质量至关重要.
- GSP基因表达受到复杂的DNA-蛋白和蛋白质-蛋白质相互作用的调节.
- 像DOF家族成员这样的转录因子在调节GSP合成中起着关键作用.
研究的目的:
- 为了识别和表征大麦SAD (Scutellum和Aleurone表达的DOF) 转录因子的小麦正态,命名为TaSAD.
- 调查TaSAD和WPBF (BPBF的小麦正经) 对GSP基因促进者的结合活性.
- 确定TaSAD和WPBF在调节小麦GSP基因表达中的转录活性.
主要方法:
- 电泳运动转移试验 (EMSA) 来评估蛋白质-DNA结合.
- 在小麦内种中进行过渡表达测试,以评估转录活性.
- 共同轰炸实验研究转录因子的协同效应.
主要成果:
- 再组合的TaSAD和WPBF蛋白与HMW-GS和LMW-GS基因促进体上的cis动机结合.
- 证实TaSAD和WPBF可以激活小麦内种中的GSP基因表达.
- 储存蛋白激活剂 (SPA) 与TaSAD或WPBF的同时表达导致了对GSP基因表达的添加效应.
结论:
- 塔萨德是大麦SAD的功能性正经体,在小麦GSP基因调节中发挥作用.
- 塔萨德和WPBF是参与小麦GSP合成的关键转录因子.
- 像TaSAD,WPBF和SPA这样的TF之间的合作互动可能进一步调节GSP表达,影响小麦质量.
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