Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Unveiling the procoagulant state in Alzheimer's disease: A novel PET imaging strategy.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Catch and relay: brighter near-infrared photoluminescence in lanthanide-based nanoparticles.

Light, science & applications·2026
Same author

Sensing with (One or Many) Upconverting Nanoparticles.

Accounts of chemical research·2026
Same author

Integrating green chemistry into SPION development: a theranostic study on prostate cell models.

Nanoscale·2026
Same author

Structural studies, thermochromic luminescent properties and crystal-to-crystal transformation of a double-stranded Cu<sup>I</sup>-I-bisquinoline coordination polymer.

RSC advances·2026
Same author

Copper-based semiconductor nanocrystals for optical applications.

Nanoscale·2026
Same journal

Amorphous High-Entropy Oxides With High-Valent Metal and Oxygen-Vacancy Pairs for Thermally Stable Catalytic Oxidation.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

H<sub>2</sub>S Self-Supplied Micelles Reverse Tumor-Immune Effector Cells Energy Metabolisms to Boost Breast Cancer Immunotherapy With Microenvironment Normalization.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Feed-Draw Printing Enables Monolithically Integrated Flexible Sensors With High Interfacial Toughness and Wide Linear Range.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Space-Time Coding Conformal Metasurfaces for Multifrequency Beam Steering and Shaping.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

3D Printing of Magnetic Soft Materials for Functional Structures and Devices.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Photothermal-Activable Artificial Macrophage With Amplified Systemic Antibacterial Responses to Combat Primary and Secondary Infection.

Advanced materials (Deerfield Beach, Fla.)·2026
查看所有相关文章

相关实验视频

Updated: Jun 14, 2026

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties
10:16

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties

Published on: January 8, 2016

14.0K

使用聚合物纳米凝进行3D光学一致性温度测量.

Tamara Muñoz-Ortiz1,2, Idoia Alayeto3, José Lifante4,5

  • 1nanomaterials for BioImaging Group (nanoBIG), Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, C/ Francisco Tomás y Valiente 7, Madrid, 28049, Spain.

Advanced materials (Deerfield Beach, Fla.)
|June 23, 2023
PubMed
概括
此摘要是机器生成的。

光学连贯温度计 (OCTh) 使用热响应纳米凝和3D成像来实现精确的,最少侵入性的温度映射. 这一突破将纳米热度计推进到潜在的临床应用.

关键词:
在3D成像中使用3D成像.纳米热度测量 (nanoothermometry) 是一种方法.光学连贯性断层扫描 (OCT)热敏的纳米凝具有热敏性.

更多相关视频

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

1.1K
Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
06:54

Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems

Published on: June 23, 2023

877

相关实验视频

Last Updated: Jun 14, 2026

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties
10:16

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties

Published on: January 8, 2016

14.0K
Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

1.1K
Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
06:54

Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems

Published on: June 23, 2023

877

科学领域:

  • 生物医学工程 生物医学工程
  • 纳米技术 纳米技术
  • 在光学成像系统中,光学成像

背景情况:

  • 纳米热度测量提供了远程,最少的侵入性温度传感,对于生物医学应用至关重要,传统方法失败.
  • 目前的纳米热度测量在临床转化方面面临限制,原因是缺乏3D热成像和依赖非标准设备.

研究的目的:

  • 开发一种新的纳米温度计技术,克服目前临床翻译的局限性.
  • 引入光学一致性温度计 (OCTh) 以同时进行3D热成像和使用临床上可用的工具.

主要方法:

  • 结合热敏聚合物纳米凝与光学一致性断层扫描 (OCT),一个标准的3D临床成像模式.
  • 使用的纳米凝体积相位过渡诱导的折射率变化作为温度敏感的OCT对比剂.
  • 验证了OCTh在经历光热过程的组织幻体中的3D热成像能力,与数值模拟进行比较.

主要成果:

  • 演示了OCTh在组织幽灵中产生3D热图像的能力.
  • 通过与数值模拟进行比较,证实了OCTh的可靠性和准确性.
  • 展示了纳米凝作为有效的温度敏感的OCT对比剂.

结论:

  • OCTh成功地克服了3D热成像和纳米热度测量的临床设备要求的障碍.
  • 这项技术为在临床环境中实施先进纳米温度计提供了可靠的基础.
  • OCTh代表了纳米热度测量用于精确温度监测的临床翻译的重要一步.