基于表型和全基因组测序数据的整合,对产生EPS的双菌株进行了表征
Dianbin Liu1, Haohan Huang1, Jinzhi Han1
1Jiangsu Key Laboratory of Immunity and Metabolism, Jiangsu International Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology/School of Stomatology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China.
Canadian journal of microbiology
|June 23, 2023
概括
从婴儿便中分离出来的Bifidobacterium longum XZM1产生具有高抗氧化活性的外聚糖 (EPS). 这种菌株显示出作为有益于肠道健康的益生菌的潜力.
科学领域:
- 微生物学 微生物学
- 益生菌 益生菌是一种益生菌.
- 我们的肠道微生物组.
背景情况:
- 双杆菌和乳杆菌物种是人类肠道微生物群的关键成员.
- 这些细菌中的外聚糖 (EPS) 与益生菌的健康益处有关.
- 识别新型EPS产生菌株对于益生菌的发展至关重要.
研究的目的:
- 为了隔离和选Bifidobacterium和Lactobacillus菌株用于EPS生产.
- 为了描述由最有生产力的菌株,Bifidobacterium longum XZM1.1.所产生的EPS.
- 评估B. longum XZM1作为益生菌的潜力.
主要方法:
- 从婴儿便中分离和选21种细菌菌株.
- 对B. longum XZM1的基因组测序和比较的基因组分析.
- 通过EPS净化,分子量测定 (凝透色谱) 和水解盐分析.
- 评估自由基清除活动.
主要成果:
- 双杆菌长XZM1表现出最高的EPS生产率.
- 基因组揭示了一个特定的基因集群,负责EPS的生产.
- 来自XZM1的EPS的分子量为4023Da,由曼诺糖,葡萄糖,银糖,阿拉比诺糖和糖组成.
- 该EPS表现出显著的氧化自由基清除能力.
结论:
- B. longum XZM1是一种高EPS产生的菌株,具有潜在的益生菌性质.
- 已识别的EPS具有抗氧化能力,有助于其有益作用.
- B. longum XZM1 是一个有希望的候选人,可以用于维持肠道平衡的益生菌应用.
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