猪养殖场废弃物中携带的与抗菌素耐药性表型相关的细菌可移植元素的表征
Priyanka Kumari Thakur1, Rajib Deb2, Seema Rani Pegu2
1ICAR-National Research Centre on Pig, Guwahati, Assam, India; All India Institute of Hygiene and Public Health, Government of India, Kolkata, West Bengal, India.
概括
在印度养猪场废物中发现了可转移的元素,包括六种特定类型. 这些移动遗传元素有助于抗生素耐药性,需要在养猪部门采取预防措施.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 环境科学 环境科学
背景情况:
- 抗生素耐药性是一个日益严重的全球健康问题.
- 已知可移植元素在抗生素耐药性的传播中起作用.
- 关于可转移元素和集成子在养猪场环境中的流行情况的研究有限.
研究的目的:
- 在印度的养猪场废物中识别和描述可转移的元素.
- 研究可移植元素在抗生素耐药性基因传播中的作用.
- 评估移动遗传元素在养猪行业传播耐药性的可能性.
主要方法:
- 从养猪场废物中鉴定了6个可转移的元素 (Tn6763,Tn6764,Tn6765,Tn2003,Tn6072,Tn6020) 在猪群中.
- 使用Klebsiella分离物 (捐赠者) 和大肠杆菌DH5α (接受者) 的结合转移实验.
- 提供者,接受者和转结合菌株的抗生素耐药性概况.
主要成果:
- 在养猪场废物样本中发现了六个可转移的元素.
- Tn6020促进了纳利迪克酸耐药性从克莱布西拉菌转移到大肠杆菌.
- 转合剂获得了纳利迪克酸和氨基醇的耐药性,这表明通过Tn6020成功转移了耐药性决定因素.
- 21.4%的耐药 Enterobacteriaceae 菌株中存在可移植元素.
结论:
- 印度养猪场废物中存在可移植元素,与抗生素耐药性有关.
- 像Tn6020这样的移动遗传元素可以在肠杆菌家族内转移抗生素耐药性基因.
- 监测和预防策略对于控制猪养殖业中抗生素耐药性的传播至关重要.
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