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在接受减肥手术的肥胖患者中氧化和DNA损伤:为期一年的随访研究

Anna Chiaramonte¹, Serena Testi¹, Caterina Pelosini²

¹Unità di Genetica, Dipartimento di Biologia, University of Pisa, Pisa, Italy.

Mutation research

|June 23, 2023

概括

肥胖会增加氧化压力和DNA损伤. 减肥手术在肥胖患者中显著降低了这些分子标记物,改善了整体健康. 在手术后监测这些变化至关重要.

科学领域:

生物化学生物化学
分子生物学分子生物学
细胞生理学细胞生理学

背景情况:

肥胖病因与氧化应激和慢性炎症有关.
脂肪组织的过剩会产生自由基,加剧炎症.
减少脂肪组织可以减轻氧化应激和炎症.

研究的目的:

评估肥胖患者细胞膜氧化和DNA损伤.
评估减肥手术对这些分子标记物的影响.
为了比较肥胖患者与正常体重的对照.

主要方法:

使用光脂质探针BODIPY® 581/591 C11进行细胞膜氧化.
使用 γH2AX 焦点测试来检测DNA双链断裂 (DSB).
在减肥手术前后,分析了正常体重和肥胖患者的外周血液淋巴细胞.

主要成果:

与正常体重对照组相比,肥胖患者的氧化细胞和DNA损伤频率 (γH2AX焦点) 显着更高.
腹部外科手术导致氧化损伤标志物的明显改善.
减肥手术后一年观察到DNA双链断裂的显著减少.

结论:

减肥手术有效地逆转肥胖引起的氧化应激和DNA损伤.
监测细胞膜氧化和DNA损伤对于肥胖患者的术后随访很重要.
这些分子标记可以作为治疗有效性和患者康复的指标.

关键词:

腹腔外科手术是什么意思在DSB中,DSB是DSB.H2AX H2AX 是一个炎症炎症是一种炎症.肥胖问题肥胖问题氧化过程中的氧化.

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