基于一种基于小鼠味觉受体细胞的新型生物传感器的乌玛米味道评价
Yuxia Fan1, Weizhu Chen1, Ninglong Zhang1
1Department of Food Science & Technology, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Biosensors & bioelectronics
|June 23, 2023
概括
使用味觉受体细胞 (TRC) 开发了一种新型生物传感器,用于检测像MSG这样的umami化合物. 这项技术提供了一种灵敏而快速的方法来评估乌玛米味道特征.
科学领域:
- 生物仿真传感器传感器
- 生物传感器技术的技术
- 味觉受体细胞研究研究
背景情况:
- 乌玛米的味觉是复杂的,并没有完全理解.
- 评估乌玛米化合物的现有方法是有限的.
- 需要先进的感官评估工具.
研究的目的:
- 开发一种自组装的生物传感器,用于检测乌玛米物质.
- 为了利用成熟的味觉受体细胞 (TRC) 作为识别元素.
- 为了快速和灵敏地评估umami特征.
主要方法:
- 隔离和培养味道干细胞以获得TRCs.
- 使用TRCs在金纳米粒子修饰的玻璃碳电极上制造生物传感器.
- 为生物传感器性能优化TRC度和化时间.
主要成果:
- 基于TRCs的生物传感器成功检测到L-单谷氨酸 (MSG) 和其他乌玛米物质.
- 观察到响应信号与MSG度 (10^-9 - 10^-5 M) 之间存在线性关系.
- 优化的参数包括10^6细胞/毫升的TRC度和12小时的潜伏时间.
结论:
- 开发的生物传感器提供了一种新的,快速和敏感的方法来检测乌玛米.
- 这项技术提供了对味道信号传导机制的洞察.
- 生物传感器显示出在智能感知系统中的应用潜力.
相关概念视频
The Physiology of Taste
4.0K
The perception of a salty flavor is facilitated by sodium ions within the oral salivary fluid. Upon consumption of a salty substance, salt crystals disassemble, leading to the liberation of its constituents—Na+ and Cl- ions. These ions subsequently dissolve into the salivary fluid present in the oral cavity. The external environment of the gustatory cells experiences an elevation in Na+ concentration, thereby establishing a potent concentration gradient. This gradient propels the...
4.0K
Gustation
48.2K
Gustation is a chemical sense that, along with olfaction (smell), contributes to our perception of taste. It starts with the activation of receptors by chemical compounds (tastants) dissolved in the saliva. The saliva and filiform papillae on the tongue distribute the tastants and increase their exposure to the taste receptors.
48.2K
Taste Buds and Receptors
2.3K
Gustation, or the sense of taste, is intrinsically linked to the anatomical structures located on the tongue. This organ's surface, along with the entirety of the oral cavity, is adorned with stratified squamous epithelium. Evident on the tongue are elevated structures known as papillae (singular = papilla), which house the mechanisms for the transduction of gustatory stimuli. Four distinct types of papillae exist, each identified by their unique morphological attributes: the circumvallate,...
2.3K


