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用于标准化排气气息采集装置的口罩的灭菌和重复使用,采用自闭包方式进行灭菌
Samuel T Shawn1, Sean W Harshman1, Christina N Davidson1
1Air Force Research Laboratory, 711th Human Performance Wing/RHBBA, 2510 Fifth Street, Area B, Building 840, Wright- Patterson AFB, OH 45433, United States of America.
在体外分析 (ReCIVA) 装置的呼吸采集器的可重复使用口罩可以通过自闭塞灭菌. 虽然重复使用引入了一些污染物,但呼吸分析结果在最多五次使用后基本上不受影响,这表明有限的重复使用是可行的.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 由于采集方法非标准化,呼气研究面临着挑战.
- 在体外分析的呼吸采集器 (ReCIVA) 提供了一种标准化的方法,但由于一次性口罩和过器而导致高成本.
- 降低这些成本对于ReCIVA在呼吸分析中的更广泛采用至关重要.
研究的目的:
- 通过自闭塞检测ReCIVA口罩和过器的灭菌和重复使用的可行性.
- 评估重复的自闭塞对口罩无菌性,过器效率和呼气化合物分析的影响.
- 确定ReCIVA消耗品的最佳重复使用限制,以平衡降低成本和数据完整性.
主要方法:
- 面具被故意污染,自化,并使用子条和光学密度测试病原体存活率.
- 复合背景分析是使用热溶解气色谱-质谱法 (TD-GC-MS) 对新型与自化口罩进行的.
- 通过引入气溶并比较过前和过后的颗粒数量来测试过器的颗粒阻断能力.
- 在胡薄荷摄入协议期间,使用新口罩和自化口罩收集了呼出的呼吸样本,用于TD-GC-MS分析.
主要成果:
- 自闭塞有效消毒污染了唾液的口罩,将病原体降低到背景水平 (p=0.2527).
- 重复的自封化增加了背景素化合物,并在五个循环后导致了显著的颗粒过器突破 (p=0.0219).
- 对呼出的气息化合物的分析 (menthone,1-Methyl-4-(1-methylethyl) -cyclohexanol,isoprene) 显示,新型口罩和5次自化口罩之间没有显著差异 (p>0.1063).
结论:
- ReCIVA的口罩和过器可以通过自动封闭进行有效的杀菌,以便重复使用.
- 建议进行有限的重复使用 (最多三次),以减轻背景污染物的增加和潜在的过器功能障碍.
- 自动封装为ReCIVA消耗品提供了具有成本效益的解决方案,促进了在呼气研究中更广泛的使用.
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