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一种模式识别受体CgTLR3参与调节Crassostrea gigas中血细胞的增殖

Jinyu Liu¹, Weilin Wang², Ning Kong³

¹Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China.

Developmental and comparative immunology

|June 23, 2023

概括

中的收费类受体 (TLR) 调节血细胞的增殖. CgTLR3与PAMPs和MyD88-2结合,影响免疫细胞反应和与增殖相关的基因表达.

科学领域:

免疫学免疫学免疫学
海洋生物学海洋生物学
细胞生物学细胞生物学

背景情况:

收费类受体 (TLRs) 对于先天性和适应性免疫至关重要,调节免疫细胞的增殖和分化.
拥有TLRs,在免疫反应中发挥作用,但它们在血细胞增殖中的特定功能需要进一步研究.

研究的目的:

研究CgTLR3对病原体相关分子模式 (PAMP) 和CgMyD88-2的结合活性.
阐明CgTLR3在调解血细胞增殖中的作用.

主要方法:

产生了CgTLR3细胞外LRR和细胞内TIR域的重组蛋白质.
使用各种PAMP和CgMyD88-2进行了结合试验.
在血细胞亚群中分析了通过qPCR和免疫光检测的CgTLR3表达.
在使用siRNA和细菌刺激抑制CgTLR3后,评估了血细胞增殖.

主要成果:

重组CgTLR3-LRR与LPS,PGN,MAN和Poly (I:C) 结合,对LPS的亲和力最高.
重组CgTLR3-TIR与rCgMyD88-2结合,其K_D值为7.22 × 10^-7.
CgTLR3主要表达在粒细胞中.
振动的刺激上调了CgTLR3mRNA的表达.
抑制CgTLR3降低了与血液形成,细胞循环和炎症因素相关的基因的表达,并降低了血细胞的增殖.

结论:

CgTLR3表现出对PAMP和MyD88-2的结合活性.
CgTLR3主要位于粒细胞中,并且在细菌感染时被上调.
通过调节关键基因表达,CgTLR3在调节血细胞增殖方面发挥着重要作用.

关键词:

这种植物是Crassostrea gigas.血细胞的增殖血细胞的增殖我的D88-2是什么意思收费像接收器一样的收费.

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