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洞察超病毒性和高度致命的Mycobacterium结核病菌株集群的病原组学和植物地理学

Igor Mokrousov^1,2, Anna Vyazovaya³, Egor Shitikov⁴

¹Laboratory of Molecular Epidemiology and Evolutionary Genetics, St. Petersburg Pasteur Institute, St. Petersburg, Russia. igormokrousov@yahoo.com.

BMC infectious diseases

|June 23, 2023

概括

一种新型的多药耐药性和高毒性Mycobacterium结核病北京14717-15集群是俄罗斯的特有病. 这项研究描述了其病原体特征,并开发了一种PCR检测方法.

科学领域:

微生物学微生物学
基因组学就是基因组学.
流行病学流行病学

背景情况:

结核菌北京基因型是一种全球流行的血统,在亚型中具有不同的临床特性.
在俄罗斯发现的M.结核病北京14717-15集群,表现为多药耐药性,高毒性和高致命性.

研究的目的:

分析M.结核病的病原体特征北京14717-15集群.
为了确定这个特定的M.结核病集群的植物地理分布.

主要方法:

从全球收集的M.结核病DNA (1996-2020) 的全基因组测序和遗传学分析.
开发和应用实时PCR试验,用于检测北京14717-15集群特定单核酸多态 (SNP).
对已识别的集群特异性突变进行基分析,以确定它们对蛋白质功能的潜在影响.

主要成果:

遗传学分析证实了北京14717-15集群在亚洲俄罗斯的特有流行,与韩国分离物有着遥远的共同祖先.
集群分离物具有耐药性突变 (RpsL Lys88Arg,KatG Ser315Thr) 并属于SIT269.9型.
在与脂质代谢,调节蛋白和细胞壁过程相关的基因中发现了可能影响蛋白质功能的新型集群特异性SNP.

结论:

成功开发了一种实时PCR方法,用于检测具有临床意义的多药耐药性和高毒性北京14717-15集群.
新发现的集群特异性突变可能有助于这种M.结核病菌株的高病毒性表型.
需要进一步的研究来阐明这些突变的病理生物学意义.

关键词:

北京基因型北京基因型药物耐药性药物耐药性药物耐药性结核病菌菌菌的结核病菌的结核病菌.病毒性病毒性整个基因组的测序.

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