使用metabarcoding和果实体库存在高度梯度中的真菌的调查方法的比较
Markus Blaschke1, Angela Siemonsmeier2,3, Janno Harjes4
1Bavarian State Institute of Forestry, Hans-Carl-von-Carlowitz-Platz 1, 85354, Freising, Germany. markus.blaschke@lwf.bayern.de.
Archives of microbiology
|June 24, 2023
概括
土壤样本的元编码揭示了比传统的果实体库存更多的真菌物种,为不同高度的真菌多样性提供了更全面的视图. 这种基于DNA的方法捕获了更广泛的真菌,包括那些隐藏或地下果实体的真菌.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生态生态学 生态生态学
- 分子生物学分子生物学
背景情况:
- 传统的真菌多样性评估依赖于果实体库存,这可能会错过神秘或地下物种.
- 环境DNA (eDNA) 的元编码为调查真菌群体提供了一种分子方法.
- 将这些方法进行比较对于理解它们在生物多样性研究中的各自优势和局限性至关重要.
研究的目的:
- 为了比较土壤metabarcoding和传统果实体库存的有效性,以评估沿海拔梯度的真菌多样性.
- 评估每个方法在检测不同真菌群体和生态协会方面的偏见和优势.
主要方法:
- 在德国巴伐利亚森林的海拔梯度上对144个地块进行了并行调查.
- 采集了用DNA测序进行真菌元编码的土壤样本.
- 进行传统的果实体清单,以记录宏观真菌的发生.
主要成果:
- 与果实体库存相比,metabarcoding检测到的真菌物种数量明显更多.
- 元编码数据显示,Basidiomycota和Ascomycota之间分布平衡,而水果机体库存则由Basidiomycota主导.
- 超编码识别了不显眼的和低温的真菌,以及较高比例的树木殖民物种具有黑色化的子,这是传统方法错过的.
结论:
- 土壤元编码提供了比传统的果实体库存更全面的真菌多样性的评估,特别是对于神秘的物种和特定的生态协会.
- 代码显示,真菌物种丰富度的高度下降,这种模式在果实体数据中并不明显.
- 两种方法都显示了相对物种社区相似性的类似模式,这表明对真菌社区结构的互补见解.
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