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Updated: Jul 25, 2025

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
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Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

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高通量电子断层扫描的协议,以初级人体血细胞中的中心离子体为例

Sebastian Köhrer1, Tobias Dittrich2, Martin Schorb3

  • 1Clinical Cooperation Unit Molecular Hematology/Oncology, German Cancer Research Center (DKFZ), and Department of Internal Medicine V, University of Heidelberg, 69120 Heidelberg, Germany; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.

STAR protocols
|June 24, 2023
PubMed
概括

这项研究引入了一种半自动电子断层扫描方法,用于在人体血细胞中高通量分析中心超结构. 这种技术显著加快了获取详细的细胞数据的速度.

关键词:
高吞吐量选 高吞吐量选显微镜的使用方法

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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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