在猪十二指肠,肝脏和肌肉组织中识别转录调节变异
Daniel Crespo-Piazuelo1, Hervé Acloque2, Olga González-Rodríguez1
1Animal Breeding and Genetics Program, IRTA, Torre Marimon, Caldes de Montbui (08140), Spain.
GigaScience
|June 24, 2023
概括
这项研究使用了猪的表达全基因组关联研究 (eGWAS) 来识别影响十二指肠,肝脏和肌肉基因表达的遗传变异. 它揭示了影响表型的数百万个重要的关联和监管元素.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 全基因组关联研究 (GWAS) 对于将遗传变异与牲畜中的表型联系起来至关重要.
- 了解十二指肠,肝脏和肌肉等关键组织的遗传调节对于动物的生产力至关重要.
研究的目的:
- 确定与猪十二指肠,肝脏和肌肉转录组中的基因表达相关的遗传多态.
- 探索 cis 和 trans 调节变体及其对基因调节的影响.
主要方法:
- 在三种品种 (Duroc,Landrace,Large White) 的300头猪上进行了GWAS表达.
- 对三种组织中数千个基因的表达进行了分析,分析了25315878种多态.
- 使用超过9.68 × 1011的关联测试来识别显著的变异基因关联.
主要成果:
- 确定了14,096,080个显著关联的变异,分为26414个表达量特征位置 (eQTL) 区域.
- 发现 cis-调节变异在转录起点的上游和3'UTRs的下游都很重要.
- 发现了39,874个影响多个基因的热点调节多态,以及丰富的序列模式.
结论:
- 这项研究提供了成千上万的表达相关变异在猪组织的全面地图.
- 这些发现揭示了调节变异影响复杂特征的分子机制.
- 公开可用的数据有助于进一步研究猪的基因型-表型关系.
相关概念视频
Master Transcription Regulators
7.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.0K
General Transcription Factors
5.4K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.4K
Transcription Factors
76.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
76.2K
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K


